Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cao, Y.Z.; Tam, S.W.; Arthur, G.; Chen, H.; Choy, P.C.
    The purification and characterization of a phospholipase A in hamster heart cytosol for the hydrolysis of phosphatidylcholine (1987), J. Biol. Chem., 262, 16927-16935.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 anti-peptide antibody
-
 Mesocricetus auratus
3.1.1.4 Sodium dodecyl sulfate
-
 Mesocricetus auratus
3.1.1.4 Triton X-100
-
 Mesocricetus auratus
3.1.1.4 Tween 20
-
 Mesocricetus auratus
3.1.1.32 rabbit anti-phospholipase A antibodies
-
 Mesocricetus auratus
3.1.1.32 SDS
-
 Mesocricetus auratus
3.1.1.32 Triton X-100
-
 Mesocricetus auratus
3.1.1.32 Tween 20
-
 Mesocricetus auratus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 0.5
-
 1-palmitoyl-2-arachidonoylphosphatidylcholine
-
 Mesocricetus auratus
3.1.1.4 0.5
-
 1-stearoyl-2-arachidonoylphosphatidylcholine
-
 Mesocricetus auratus
3.1.1.32 0.5
-
 1-palmitoyl-2-arachidonoylglycerophosphocholine
-
 Mesocricetus auratus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 cytoplasm
-
 Mesocricetus auratus 5737
-
3.1.1.32 cytosol
-
 Mesocricetus auratus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.32 Ca2+ 10 mM, 10% activation Mesocricetus auratus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 14000
-
 SDS-PAGE, Mr of one subunit Mesocricetus auratus
3.1.1.4 140000
-
 gel filtration, multimeric complex Mesocricetus auratus
3.1.1.32 14000
-
 x * 14000, at least six subunits of the native enzyme can be cross-linked, SDS-PAGE Mesocricetus auratus
3.1.1.32 140000
-
 gel filtration Mesocricetus auratus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Mesocricetus auratus cardiac contraction and excitation ?
-
 ?
3.1.1.32 additional information Mesocricetus auratus the enzyme is important in phospholipid catabolism and the remodeling of the acyl groups of phospholipids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Mesocricetus auratus
-
-
-
3.1.1.32 Mesocricetus auratus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 ion-exchange chromatography, gel-filtration chromatography,167fold Mesocricetus auratus
3.1.1.32
-
 Mesocricetus auratus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 heart
-
 Mesocricetus auratus
-
3.1.1.32 heart
-
 Mesocricetus auratus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 0.0192
-
 substrate: phosphatidylcholine Mesocricetus auratus
3.1.1.32 1.155
-
-
 Mesocricetus auratus

Storage Stability

EC Number Storage Stability Organism
3.1.1.4 -20°C, 50 mM Hepes, 5 mM EDTA, 48 hours without loss of activity, 75% of the initial activity remains after one week, storage at -70°C does not improve the enzymes stability Mesocricetus auratus
3.1.1.32 -20°C, 50 mM Hepes, 5 mM EDTA, pH 7.4, stable for 48 h, 25% loss of activity after 1 week Mesocricetus auratus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O
-
 Mesocricetus auratus 1-palmitoyl-glycerophosphorylcholine + arachidonic acid
-
 ?
3.1.1.4 1-palmitoyl-2-linoleoylphosphatidylcholine + H2O
-
 Mesocricetus auratus 1-palmitoyl-glycerophosphorylcholine + linolic acid
-
 ?
3.1.1.4 1-stearoyl-2-arachidonoylphosphatidylcholine + H2O
-
 Mesocricetus auratus 1-stearoyl-glycerophosphorylcholine + arachidonic acid
-
 ?
3.1.1.4 phosphatidylcholine + H2O
-
 Mesocricetus auratus 1-acylglycerophosphocholine + fatty acid
-
 ?
3.1.1.4 phosphatidylethanolamine + H2O
-
 Mesocricetus auratus 1-acylglycerophosphorylethanolamine + fatty acid
-
 ?
3.1.1.4 phospholipids + H2O cardiac contraction and excitation Mesocricetus auratus ?
-
 ?
3.1.1.32 1-palmitoyl-2-arachidonoylglycerophosphocholine + H2O
-
 Mesocricetus auratus palmitic acid + 2-arachidonoylglycerophosphocholine
-
 ?
3.1.1.32 1-stearoyl-2-arachidonoylglycerophosphocholine + H2O
-
 Mesocricetus auratus stearic acid + 2-arachidonoylglycerophosphocholine
-
 ?
3.1.1.32 1-stearoyl-2-arachidonoylglycerophosphoinositol + H2O
-
 Mesocricetus auratus stearic acid + 2-arachidonoylglycerophosphoinositol
-
 ?
3.1.1.32 additional information the enzyme exhibits both phospholipase A1 and phospholipase A2 activity Mesocricetus auratus ?
-
 ?
3.1.1.32 additional information the enzyme is important in phospholipid catabolism and the remodeling of the acyl groups of phospholipids Mesocricetus auratus ?
-
 ?
3.1.1.32 phosphatidylcholine + H2O
-
 Mesocricetus auratus ?
-
 ?
3.1.1.32 phosphatidylethanolamine + H2O
-
 Mesocricetus auratus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.4 hexamer 6 * 14000, identical subunits, at least six subunits could be cross-linked Mesocricetus auratus
3.1.1.32 ? x * 14000, at least six subunits of the native enzyme can be cross-linked, SDS-PAGE Mesocricetus auratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 9
-
 substrate: 1-palmitoyl-2-arachidonoylphosphatidylcholine Mesocricetus auratus
3.1.1.32 9
-
-
 Mesocricetus auratus