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Literature summary extracted from
- Biochemical characterization of the restriction-modification system of Bacillus sphaericus (1978), Eur. J. Biochem., 89, 523-529.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.21.4 | in dilute solutions at 37°C, 50% inactivation after 30 min | Lysinibacillus sphaericus |
| 3.1.21.4 | inactivation by lyophilization | Lysinibacillus sphaericus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.21.4 | high ionic strength | irreversible inactivation | Lysinibacillus sphaericus | |
| 3.1.21.4 | Zn2+ | 2 mM, complete inhibition | Lysinibacillus sphaericus |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.21.4 | Mg2+ | required | Lysinibacillus sphaericus | |
| 3.1.21.4 | Mn2+ | can partially replace Mg2+, optimal concentration is 10 mM | Lysinibacillus sphaericus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.4 | 35000 | - |
gel filtration | Lysinibacillus sphaericus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.4 | Lysinibacillus sphaericus | - |
enzyme endoR*Bsp | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.21.4 | - |
Lysinibacillus sphaericus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.21.4 | -20°C, 50% glycerol, 8-12 months no appreciable loss of activity | Lysinibacillus sphaericus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.4 | DNA + H2O | single strandede DNA and double stranded DNA | Lysinibacillus sphaericus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.21.4 | monomer | 1 * 35000, SDS-PAGE | Lysinibacillus sphaericus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.21.4 | endoR*Bsp | - |
Lysinibacillus sphaericus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.4 | 8.2 | - |
- |
Lysinibacillus sphaericus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.4 | 5 | 10 | some activity is detected even at pH 5 and pH 10 | Lysinibacillus sphaericus |
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Release 2023.1 (January 2023)
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