Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kvaratskhelia, M.; White, M.F.
    An archaeal holliday junction resolving enzyme from Sulfolobus solfataricus exhibits unique properties (2000), J. Mol. Biol., 295, 193-202.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.10 Mg2+ optimum: 15-30 mM MgCl2, 10% of maximal activity in 4 mM MgCl2 Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.10 DNA + H2O Saccharolobus solfataricus holliday structure hydrolyzed DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.10 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.10 partial purification Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.10 DNA + H2O introduces paired nicks into opposing continous strands of a stacked X-junction, independent of local sequence, cleaves an Y-junction in the presence of a bulge in one strand so that stacking of the junction becomes possible Saccharolobus solfataricus hydrolyzed DNA
-
 ?
3.1.21.10 DNA + H2O holliday structure Saccharolobus solfataricus hydrolyzed DNA
-
 ?
3.1.21.10 Holliday junction 3 in DNA + H2O
-
 Saccharolobus solfataricus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.10 Hje endonuclease
-
 Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.21.10 0.00014
-
 Holliday junction 3 in DNA at 50°C Saccharolobus solfataricus
3.1.21.10 0.02
-
 Holliday junction 3 in DNA prediction: at 80°C Saccharolobus solfataricus