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Literature summary extracted from

  • Sogin, M.L.; Page, B.; Pace, N.R.
    Partial purification and properties of a ribosomal RNA maturation endonuclease from Bacillus subtilis (1977), J. Biol. Chem., 252, 1350-1357.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.26.8 NH4+ activates, inhibition above 0.1 M Bacillus subtilis
3.1.26.8 putrescine divalent cation, metal or polyamine is required, 0.01 M putrescine is as effective as Mg2+ Bacillus subtilis
3.1.26.8 spermidine divalent cation, metal or polyamine is required, 0.3 mM is as effective as Mg2+ Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.8 Zn2+ inhibits in presence of Mg2+ Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.8 Ca2+ divalent cation, metal or polyamine is required Bacillus subtilis
3.1.26.8 K+ activates, inhibition above 0.1 M Bacillus subtilis
3.1.26.8 Mg2+ divalent cation, metal or polyamine is required Bacillus subtilis
3.1.26.8 Mn2+ divalent cation, metal or polyamine is required Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.26.8 5S rRNA precursor + H2O Bacillus subtilis responsible for terminal maturation of 5S rRNA ?
-
 ?
3.1.26.8 5S rRNA precursor + H2O Bacillus subtilis 168 responsible for terminal maturation of 5S rRNA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.8 Bacillus subtilis
-
-
-
3.1.26.8 Bacillus subtilis 168
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.8 partial Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.8 5S rRNA precursor + H2O the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA Bacillus subtilis mature 5S rRNA + oligonucleotides the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA ?
3.1.26.8 5S rRNA precursor + H2O the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA Bacillus subtilis 168 mature 5S rRNA + oligonucleotides the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA ?
3.1.26.8 5S rRNA precursor + H2O responsible for terminal maturation of 5S rRNA Bacillus subtilis ?
-
 ?
3.1.26.8 5S rRNA precursor + H2O responsible for terminal maturation of 5S rRNA Bacillus subtilis 168 ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.8 7 8.5
-
 Bacillus subtilis