Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Haas, H.; Redl, B.; Leitner, E.; Stöffler, G.
    Penicillium chrysogenum extracellular acid phosphatase: purification and biochemical characterization (1991), Biochim. Biophys. Acta, 1074, 392-397.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Hg2+
-
 Penicillium chrysogenum
3.1.3.2 phosphate competitive Penicillium chrysogenum
3.1.3.2 Phosphomycin ,10 mM, 25% inhibition Penicillium chrysogenum
3.1.3.2 SDS
-
 Penicillium chrysogenum
3.1.3.2 Zn2+
-
 Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 50000
-
 gel filtration Penicillium chrysogenum
3.1.3.2 57000
-
 1 * 57000, SDS-PAGE Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Penicillium chrysogenum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein contains 12% carbohydrate Penicillium chrysogenum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Penicillium chrysogenum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 culture medium
-
 Penicillium chrysogenum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 364
-
-
 Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Penicillium chrysogenum p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 monomer 1 * 57000, SDS-PAGE Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 60
-
-
 Penicillium chrysogenum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.2 10 60 10°C: 20% of maximal activity, 30°C: 45% of maximal activity, 60°C: optimum Penicillium chrysogenum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 60
-
 27% loss of activity after 5 min, 33% loss of activity after 10 min, 67% loss of activity after 15 min Penicillium chrysogenum
3.1.3.2 70
-
 10 min, complete inactivation Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.5
-
-
 Penicillium chrysogenum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 4.5 6.5 pH 4.5: about 30% of maximal activity, pH 6.5: about 15% of maximal activity Penicillium chrysogenum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.2 3.5
-
 loss of activity below Penicillium chrysogenum