Any feedback?
Literature summary extracted from
- Purification and properties of phospholipase D from Streptomyces antibioticus (1993), Biosci. Biotechnol. Biochem., 57, 1946-1948.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | 2-mercaptoethanol | - |
Streptomyces antibioticus |  |
| 3.1.4.4 | dithiothreitol | - |
Streptomyces antibioticus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 64000 | - |
chromatofocusing, SDS-PAGE | Streptomyces antibioticus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | phospholipid + alcohol | Streptomyces antibioticus | transphosphaditylation | phospholipid + alcohol | - |
? | |
| 3.1.4.4 | phospholipid + H2O | Streptomyces antibioticus | phosphoric ester hydrolysis | phosphatidic acid + alcohol | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | Streptomyces antibioticus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.4 | to homogeneity | Streptomyces antibioticus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | culture supernatant | - |
Streptomyces antibioticus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 1437 | - |
- |
Streptomyces antibioticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | lysophosphatidylcholine + H2O | - |
Streptomyces antibioticus | monoacylglycerophosphate + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + D-arabinose | - |
Streptomyces antibioticus | phosphatidylarabinose + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + D-fructose | - |
Streptomyces antibioticus | phosphatidylfructose + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + D-galactose | - |
Streptomyces antibioticus | phosphatidylgalactose + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + D-glucose | - |
Streptomyces antibioticus | phosphatidylglucose + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + D-mannose | - |
Streptomyces antibioticus | phosphatidylmannose + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + D-xylose | - |
Streptomyces antibioticus | phosphatidylxylose + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + diethyleneglycol | - |
Streptomyces antibioticus | phosphatidyldiethyleneglycol + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + diethyleneglycol monomethyl ester | - |
Streptomyces antibioticus | diethyleneglycol dimethyl phosphatidic acid + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + ethyleneglycol | - |
Streptomyces antibioticus | phosphatidylethyleneglycol + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + ethyleneglycol monomethyl ester | - |
Streptomyces antibioticus | ethyleneglycol monomethyl phosphatidic acid + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + H2O | - |
Streptomyces antibioticus | 1,2-diacylglycerophosphate + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + L-sorbose | - |
Streptomyces antibioticus | phosphatidylsorbose + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + triethyleneglycol | - |
Streptomyces antibioticus | phosphatidyltriethyleneglycol + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + triethyleneglycol monomethyl ester | - |
Streptomyces antibioticus | triethyleneglycol trimethyl phosphatidic acid + choline | - |
? | |
| 3.1.4.4 | phosphatidylethanolamine + H2O | - |
Streptomyces antibioticus | 1,2-diacylglycerophosphate + ethanolamine | - |
r | |
| 3.1.4.4 | phospholipid + alcohol | transphosphaditylation | Streptomyces antibioticus | phospholipid + alcohol | - |
? | |
| 3.1.4.4 | phospholipid + H2O | phosphoric ester hydrolysis | Streptomyces antibioticus | phosphatidic acid + alcohol | - |
? | |
| 3.1.4.4 | sphingomyelin + H2O | - |
Streptomyces antibioticus | N-acylsphingosylphosphate + choline | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 60 | 70 | - |
Streptomyces antibioticus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 5.5 | - |
- |
Streptomyces antibioticus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 4 | 8 | stable after 2 h at 37°C | Streptomyces antibioticus |
| 3.1.4.4 | 5.5 | - |
50% loss of activity after 30 min at 70°C | Streptomyces antibioticus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
