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Literature summary extracted from
- A new type of Streptomycete arylsulfatase with high affinity to the sulfuryl moiety of the substrate (1995), Biosci. Biotechnol. Biochem., 59, 1069-1075.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.6.1 | medicine | the enzyme can be accepted as the enzyme component of antitumor antibody-enzyme conjugates for target chemotherapy | Streptomyces sp. |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.1 | (NH4)2SO4 | - |
Streptomyces sp. |  |
| 3.1.6.1 | 1,10-phenanthroline | - |
Streptomyces sp. | |
| 3.1.6.1 | 2-mercaptoethanol | - |
Streptomyces sp. | |
| 3.1.6.1 | AlCl3 | - |
Streptomyces sp. | |
| 3.1.6.1 | BaCl2 | - |
Streptomyces sp. | |
| 3.1.6.1 | citrate | - |
Streptomyces sp. | |
| 3.1.6.1 | CoCl2 | - |
Streptomyces sp. | |
| 3.1.6.1 | CuCl2 | - |
Streptomyces sp. | |
| 3.1.6.1 | Cys | - |
Streptomyces sp. | |
| 3.1.6.1 | dithiothreitol | - |
Streptomyces sp. | |
| 3.1.6.1 | EDTA | - |
Streptomyces sp. | |
| 3.1.6.1 | FeCl2 | - |
Streptomyces sp. | |
| 3.1.6.1 | Hg2+ | HgCl2 | Streptomyces sp. | |
| 3.1.6.1 | imidazole | - |
Streptomyces sp. | |
| 3.1.6.1 | iodoacetate | - |
Streptomyces sp. | |
| 3.1.6.1 | K2HPO4 | - |
Streptomyces sp. | |
| 3.1.6.1 | K2SO3 | - |
Streptomyces sp. | |
| 3.1.6.1 | KCl | - |
Streptomyces sp. | |
| 3.1.6.1 | MnCl2 | - |
Streptomyces sp. | |
| 3.1.6.1 | NaCl | - |
Streptomyces sp. | |
| 3.1.6.1 | NEM | - |
Streptomyces sp. | |
| 3.1.6.1 | NiCl2 | - |
Streptomyces sp. | |
| 3.1.6.1 | p-(amidinophenyl)methanesulfonyl fluoride HCl | - |
Streptomyces sp. | |
| 3.1.6.1 | PCMB | - |
Streptomyces sp. | |
| 3.1.6.1 | SO42- | K2SO4 | Streptomyces sp. | |
| 3.1.6.1 | Zn2+ | ZnCl2 | Streptomyces sp. | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.1 | Ca2+ | required | Streptomyces sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 46500 | - |
1 * 46500, SDS-PAGE | Streptomyces sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.6.1 | Streptomyces sp. | - |
rylsulfatase Es-2 | - |
| 3.1.6.1 | Streptomyces sp. T109-3 | - |
rylsulfatase Es-2 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.6.1 | arylsulfatase Es-2 | Streptomyces sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | additional information | - |
- |
Streptomyces sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.1 | 2'',3''-di-O-acetyletoposide 4'-sulfate + H2O | - |
Streptomyces sp. | 2'',3''-di-O-acetyletoposide + sulfate | - |
? | |
| 3.1.6.1 | 4-acetylphenyl sulfate + H2O | - |
Streptomyces sp. | 4-acetylphenol + sulfate | - |
? | |
| 3.1.6.1 | 4-acetylphenyl sulfate + H2O | - |
Streptomyces sp. T109-3 | 4-acetylphenol + sulfate | - |
? | |
| 3.1.6.1 | 4-methylumbelliferyl sulfate + H2O | - |
Streptomyces sp. | 4-methylumbelliferone + sulfate | - |
? | |
| 3.1.6.1 | 4-methylumbelliferyl sulfate + H2O | - |
Streptomyces sp. T109-3 | 4-methylumbelliferone + sulfate | - |
? | |
| 3.1.6.1 | 4-nitrocatechol sulfate + H2O | - |
Streptomyces sp. | 4-nitrocatechol + sulfate | - |
? | |
| 3.1.6.1 | 4-nitrocatechol sulfate + H2O | - |
Streptomyces sp. T109-3 | 4-nitrocatechol + sulfate | - |
? | |
| 3.1.6.1 | 4-nitrophenyl sulfate + H2O | - |
Streptomyces sp. | 4-nitrophenol + sulfate | - |
? | |
| 3.1.6.1 | 4-nitrophenyl sulfate + H2O | - |
Streptomyces sp. T109-3 | 4-nitrophenol + sulfate | - |
? | |
| 3.1.6.1 | chondroitin sulfate C + H2O | - |
Streptomyces sp. | chondroitin + sulfate | - |
? | |
| 3.1.6.1 | chondroitin sulfate C + H2O | - |
Streptomyces sp. T109-3 | chondroitin + sulfate | - |
? | |
| 3.1.6.1 | etoposide 4'-sulfate + H2O | - |
Streptomyces sp. | etoposide + sulfate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.6.1 | monomer | 1 * 46500, SDS-PAGE | Streptomyces sp. |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.6.1 | arylsulfatase Es-2 | - |
Streptomyces sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 30 | - |
enzyme Es-2 | Streptomyces sp. |
| 3.1.6.1 | 37 | - |
enzyme Es-1 | Streptomyces sp. |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 4 | 50 | 4°C: about 40% of maximal activity, 50°C: about 25% of maximal activity, enzyme ES-2 | Streptomyces sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 30 | - |
enzyme Es-2, stable below | Streptomyces sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 9 | - |
enzyme ES-2 | Streptomyces sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 7.5 | 10 | pH 7.5: about 30% of maximal activity, pH 10: about 90% of maximal activity, enzyme Es-2 | Streptomyces sp. |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.1 | 5.5 | - |
rapid loss of activity below | Streptomyces sp. |
| 3.1.6.1 | 8 | 9.5 | enzyme ES-2, stable | Streptomyces sp. |
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