EC Number | General Stability | Organism |
---|---|---|
3.2.1.24 | stable in the presence of Co2+ at 37°C | Rattus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.24 | Cu2+ | strong inhibition at 1 mM | Rattus sp. | |
3.2.1.24 | EDTA | strong inhibition at 1 mM | Rattus sp. | |
3.2.1.24 | Fe2+ | slight inhibition at 1 mM | Rattus sp. | |
3.2.1.24 | iodoacetamide | slight inhibition at 1 mM | Rattus sp. | |
3.2.1.24 | p-chloromercuribenzenesulfonic acid | strong inhibition at 1 mM | Rattus sp. | |
3.2.1.24 | swainsonine | 50% inhibition in the range of 0.3-0.6 mM | Rattus sp. | |
3.2.1.24 | Zn2+ | strong inhibition at 1 mM | Rattus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.24 | 0.065 | - |
Man5GlcNAc | - |
Rattus sp. | |
3.2.1.24 | 0.11 | - |
Man9GlcNAc | - |
Rattus sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.24 | membrane | from microsomal membranes | Rattus sp. | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.24 | Co2+ | 20% activation at 1 mM | Rattus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.24 | 110000 | - |
2 * 110000, reducing and non-reducing SDS-PAGE | Rattus sp. |
3.2.1.24 | 220000 | - |
gel filtration | Rattus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.24 | alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + 6 H2O | Rattus sp. | - |
6 alpha-D-mannopyranose + alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc | end products | ? | |
3.2.1.24 | Man6GlcNAc + H2O | Rattus sp. | ordered hydrolysis of alpha(1-2)-residues followed by hydrolysis of alpha(1-3)- and alpha(1-6)-linked residues | alpha-D-mannopyranose + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.24 | Rattus sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.24 | to homogeneity, chromatography steps | Rattus sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.24 | liver | - |
Rattus sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.24 | 16.27 | - |
- |
Rattus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.24 | alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + 6 H2O | - |
Rattus sp. | 6 alpha-D-mannopyranose + alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc | end products | ? | |
3.2.1.24 | Man6GlcNAc + H2O | ordered hydrolysis of alpha(1-2)-residues followed by hydrolysis of alpha(1-3)- and alpha(1-6)-linked residues | Rattus sp. | alpha-D-mannopyranose + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.24 | dimer | 2 * 110000, reducing and non-reducing SDS-PAGE | Rattus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.24 | 6.1 | 6.5 | - |
Rattus sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.24 | 0.3 | 0.6 | swainsonine | - |
Rattus sp. |