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Literature summary extracted from

  • Manjunath, P.; Shenoy, B.C.; Raghavendra Roa, M.R.
    Fungal glucoamylases (1983), J. Appl. Biochem., 5, 235-260.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 48000
-
 glucoamylase I, gel filtration Coniophora cerebella
3.2.1.3 48000
-
 glucoamylase I, approach to equilibrium method, gel filtration Rhizopus arrhizus
3.2.1.3 49000
-
 glucoamylase II, approach to equilibrium method Mucor rouxianus
3.2.1.3 54000
-
-
 Aspergillus oryzae
3.2.1.3 54000
-
 glucoamylase II, approach to equilibrium method, gel filtration Aspergillus foetidus
3.2.1.3 55000
-
 glucoamylase I, gel filtration Endomyces sp.
3.2.1.3 59000
-
 glucoamylase I, approach to equilibrium method Mucor rouxianus
3.2.1.3 60000
-
 x * 60000, glucoamylase II, SDS-PAGE Aspergillus candidus
3.2.1.3 60000
-
 1 * 60000, glucoamylase II, SDS-PAGE Aspergillus foetidus
3.2.1.3 62000
-
 glucoamylase I, gel filtration Aspergillus phoenicis
3.2.1.3 67500
-
 1 * 67500, SDS-PAGE Aspergillus awamori
3.2.1.3 69000
-
-
 Acremonium charticola
3.2.1.3 69000
-
 glucoamylase I, calculation from sedimentation and diffusion data Aspergillus oryzae
3.2.1.3 70000
-
 x * 70000, glucoamylase M2, SDS-PAGE Aspergillus phoenicis
3.2.1.3 71600
-
 gel filtration Aspergillus awamori
3.2.1.3 75000
-
 glucoamylase I, approach to equilibrium method, gel filtration Aspergillus foetidus
3.2.1.3 75000
-
 x * 75000, glucoamylase I, SDS-PAGE Aspergillus candidus
3.2.1.3 78000
-
 1 * 78000, glucoamylase I, SDS-PAGE Aspergillus foetidus
3.2.1.3 90000
-
 x * 90000, glucoamylase I, SDS-PAGE Aspergillus phoenicis
3.2.1.3 100000
-
 glycoamylase I, density gradient ultracentrifugation Rhizopus arrhizus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Acremonium charticola
-
-
-
3.2.1.3 Amorphotheca resinae
-
-
-
3.2.1.3 Aspergillus awamori
-
-
-
3.2.1.3 Aspergillus candidus
-
-
-
3.2.1.3 Aspergillus foetidus
-
-
-
3.2.1.3 Aspergillus niger
-
-
-
3.2.1.3 Aspergillus oryzae
-
-
-
3.2.1.3 Aspergillus phoenicis
-
-
-
3.2.1.3 Coniophora cerebella
-
-
-
3.2.1.3 Endomyces sp.
-
-
-
3.2.1.3 Mucor rouxianus
-
-
-
3.2.1.3 Pyricularia grisea
-
-
-
3.2.1.3 Rhizopus arrhizus
-
-
-
3.2.1.3 Thermomyces lanuginosus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein
-
 Aspergillus oryzae
3.2.1.3 glycoprotein
-
 Mucor rouxianus
3.2.1.3 glycoprotein
-
 Aspergillus awamori
3.2.1.3 glycoprotein
-
 Acremonium charticola
3.2.1.3 glycoprotein enzyme contains 32 mol of carbohydrate per mol of enzyme Rhizopus arrhizus
3.2.1.3 glycoprotein carbohydrate groups seem to play an important role in stabilizing the structure against inactivation by heat and storage Aspergillus niger
3.2.1.3 glycoprotein carbohydrate groups seem to play an important role in stabilizing the structure against inactivation by heat and storage Rhizopus arrhizus
3.2.1.3 glycoprotein carbohydrate groups seem to play an important role in stabilizing the structure against inactivation by heat and storage Aspergillus phoenicis
3.2.1.3 glycoprotein carbohydrate groups seem to play an important role in stabilizing the structure against inactivation by heat and storage Aspergillus candidus
3.2.1.3 glycoprotein carbohydrate groups seem to play an important role in stabilizing the structure against inactivation by heat and storage Aspergillus foetidus
3.2.1.3 glycoprotein glucoamylase I and glucoamylase II contain 53.0 mol of carbohydrate per mol of enzyme Aspergillus candidus
3.2.1.3 glycoprotein glucoamylase II contains 40 mol of carbohydrate per mol of enzyme Aspergillus phoenicis
3.2.1.3 glycoprotein glucoamylase I contains 52.2 mol of carbohydrate per mol of enzyme, glucoamylase II contains 48.5 mol of carbohydrate per mol of enzyme Aspergillus foetidus
3.2.1.3 glycoprotein enzyme contains 89 mol of carbohydrate per mol of enzyme Rhizopus arrhizus
3.2.1.3 glycoprotein glucoamylase M1 contains 102 mol of carbohydrate per mol of enzyme, glucoamylase M2 contains 57 mol of carbohydrate per mol of enzyme Aspergillus phoenicis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 3-alpha-maltosylglucose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 4-alpha-nigerosyl-glucose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 6-alpha-maltosylglucose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 amylodextrin + H2O amylodextrin DP-15 Rhizopus arrhizus ?
-
 ?
3.2.1.3 amylopectin + H2O
-
 Aspergillus niger D-glucose + ?
-
 ?
3.2.1.3 amylopectin + H2O
-
 Rhizopus arrhizus D-glucose + ?
-
 ?
3.2.1.3 amylopectin + H2O
-
 Aspergillus oryzae D-glucose + ?
-
 ?
3.2.1.3 amylopectin + H2O
-
 Aspergillus phoenicis D-glucose + ?
-
 ?
3.2.1.3 amylopectin + H2O
-
 Mucor rouxianus D-glucose + ?
-
 ?
3.2.1.3 amylopectin + H2O
-
 Aspergillus awamori D-glucose + ?
-
 ?
3.2.1.3 amylopectin + H2O
-
 Acremonium charticola D-glucose + ?
-
 ?
3.2.1.3 amylose + H2O
-
 Aspergillus niger glucose + ?
-
 ?
3.2.1.3 amylose + H2O
-
 Rhizopus arrhizus glucose + ?
-
 ?
3.2.1.3 amylose + H2O
-
 Aspergillus oryzae glucose + ?
-
 ?
3.2.1.3 amylose + H2O
-
 Aspergillus phoenicis glucose + ?
-
 ?
3.2.1.3 amylose + H2O
-
 Mucor rouxianus glucose + ?
-
 ?
3.2.1.3 amylose + H2O
-
 Aspergillus awamori glucose + ?
-
 ?
3.2.1.3 amylose + H2O
-
 Acremonium charticola glucose + ?
-
 ?
3.2.1.3 dextrin + H2O
-
 Aspergillus phoenicis ?
-
 ?
3.2.1.3 dextrin + H2O beta-limit dextrin Aspergillus niger ?
-
 ?
3.2.1.3 dextrin + H2O beta-limit dextrin Rhizopus arrhizus ?
-
 ?
3.2.1.3 dextrin + H2O beta-limit dextrin Aspergillus oryzae ?
-
 ?
3.2.1.3 isomaltopentaose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 isomaltopentaose + H2O
-
 Aspergillus awamori ?
-
 ?
3.2.1.3 isomaltose + H2O
-
 Aspergillus niger glucose
-
 ?
3.2.1.3 isomaltose + H2O
-
 Rhizopus arrhizus glucose
-
 ?
3.2.1.3 isomaltose + H2O
-
 Aspergillus phoenicis glucose
-
 ?
3.2.1.3 isomaltose + H2O
-
 Aspergillus awamori glucose
-
 ?
3.2.1.3 isomaltotetraose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 isomaltotetraose + H2O
-
 Aspergillus awamori ?
-
 ?
3.2.1.3 isomaltotriose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 isomaltotriose + H2O
-
 Aspergillus awamori ?
-
 ?
3.2.1.3 maltopentaose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 maltopentaose + H2O
-
 Rhizopus arrhizus ?
-
 ?
3.2.1.3 maltopentaose + H2O
-
 Aspergillus oryzae ?
-
 ?
3.2.1.3 maltopentaose + H2O
-
 Aspergillus awamori ?
-
 ?
3.2.1.3 maltose + H2O
-
 Aspergillus niger 2 glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Rhizopus arrhizus 2 glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Aspergillus oryzae 2 glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Aspergillus phoenicis 2 glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Mucor rouxianus 2 glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Aspergillus awamori 2 glucose
-
 ?
3.2.1.3 maltotetraose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 maltotetraose + H2O
-
 Rhizopus arrhizus ?
-
 ?
3.2.1.3 maltotetraose + H2O
-
 Aspergillus oryzae ?
-
 ?
3.2.1.3 maltotetraose + H2O
-
 Aspergillus awamori ?
-
 ?
3.2.1.3 maltotetraose + H2O
-
 Acremonium charticola ?
-
 ?
3.2.1.3 maltotriose + H2O
-
 Aspergillus niger maltose + glucose
-
 ?
3.2.1.3 maltotriose + H2O
-
 Rhizopus arrhizus maltose + glucose
-
 ?
3.2.1.3 maltotriose + H2O
-
 Aspergillus oryzae maltose + glucose
-
 ?
3.2.1.3 maltotriose + H2O
-
 Aspergillus phoenicis maltose + glucose
-
 ?
3.2.1.3 maltotriose + H2O
-
 Mucor rouxianus maltose + glucose
-
 ?
3.2.1.3 maltotriose + H2O
-
 Aspergillus awamori maltose + glucose
-
 ?
3.2.1.3 maltotriose + H2O
-
 Acremonium charticola maltose + glucose
-
 ?
3.2.1.3 methyl-alpha-D-glucoside + H2O
-
 Aspergillus niger methanol + alpha-D-glucose
-
 ?
3.2.1.3 nigerose + H2O
-
 Aspergillus niger 2 alpha-D-glucose
-
 ?
3.2.1.3 nigerose + H2O
-
 Aspergillus awamori 2 alpha-D-glucose
-
 ?
3.2.1.3 panose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 panose + H2O
-
 Rhizopus arrhizus ?
-
 ?
3.2.1.3 panose + H2O
-
 Aspergillus awamori ?
-
 ?
3.2.1.3 phenyl alpha-glucoside + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 sucrose + H2O
-
 Aspergillus niger D-glucose + D-fructose
-
 ?
3.2.1.3 trehalose + H2O
-
 Aspergillus niger D-glucose
-
 ?
3.2.1.3 turanose + H2O
-
 Aspergillus niger D-fructose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 ?
-
 Rhizopus arrhizus
3.2.1.3 ?
-
 Mucor rouxianus
3.2.1.3 ? x * 90000, glucoamylase I, SDS-PAGE Aspergillus phoenicis
3.2.1.3 ? x * 75000, glucoamylase I, SDS-PAGE Aspergillus candidus
3.2.1.3 ? x * 70000, glucoamylase M2, SDS-PAGE Aspergillus phoenicis
3.2.1.3 ? x * 60000, glucoamylase II, SDS-PAGE Aspergillus candidus
3.2.1.3 monomer
-
 Rhizopus arrhizus
3.2.1.3 monomer
-
 Aspergillus oryzae
3.2.1.3 monomer
-
 Aspergillus candidus
3.2.1.3 monomer 1 * 78000, glucoamylase I, SDS-PAGE Aspergillus foetidus
3.2.1.3 monomer 1 * 67500, SDS-PAGE Aspergillus awamori
3.2.1.3 monomer 1 * 60000, glucoamylase II, SDS-PAGE Aspergillus foetidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 5 5.5
-
 Aspergillus oryzae
3.2.1.3 5 5.5
-
 Acremonium charticola
3.2.1.3 6.6
-
-
 Thermomyces lanuginosus