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Literature summary extracted from

  • Chen, W.P.; Matsuo, M.; Yasui, T.
    Purification and some properties of beta-1,3-xylanase from Aspergillus terreus A-07 (1986), Agric. Biol. Chem., 50, 1183-1194.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.32 Hg2+ 1 mM, strong inhibition Aspergillus terreus
3.2.1.32 Mn2+ 1 mM, strong inhibition Aspergillus terreus
3.2.1.32 N-bromosuccinimide 1 mM, strong inhibition Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.32 Aspergillus terreus
-
 A-07
-
3.2.1.32 Aspergillus terreus A-07
-
 A-07
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.32 6 different enzyme forms: EF-1, EF-2, EF-3, EF-4, EF-5 and EF-6 Aspergillus terreus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.32 6.4
-
 enzyme form EF-3 and EF-5 Aspergillus terreus
3.2.1.32 8.5
-
 enzyme form EF-4 Aspergillus terreus
3.2.1.32 9.3
-
 enzyme form EF-2 Aspergillus terreus
3.2.1.32 13.3
-
 enzyme form EF-6 Aspergillus terreus
3.2.1.32 15.2
-
 enzyme form EF-1 Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.32 beta-1,3-xylan + H2O action pattern of type I occurs in the hydrolysis by enzyme form EF-1, EF-2 and EF-5: beta-1,3-xylobiose, beta-1,3-xylotriose, beta-1,3-xylotetraose and an unknown disaccharide are liberated. The action pattern of type II occurs during hydrolysis by enzyme form EF-4 and EF-6. In the initial stage the enzyme produces mainly beta-1,2-xylobiose, beta-1,3-xylotriose and beta-1,3-xylotetraose. As hydrolysis proceeds an isomeric xylotriose is detectable as well as xylose, beta-1,3-xylobiose, beta-1,3-xylotriose and beta-1,3-xylotetraose Aspergillus terreus xylose + xylooligosaccharides
-
 ?
3.2.1.32 beta-1,3-xylan + H2O action pattern of type I occurs in the hydrolysis by enzyme form EF-1, EF-2 and EF-5: beta-1,3-xylobiose, beta-1,3-xylotriose, beta-1,3-xylotetraose and an unknown disaccharide are liberated. The action pattern of type II occurs during hydrolysis by enzyme form EF-4 and EF-6. In the initial stage the enzyme produces mainly beta-1,2-xylobiose, beta-1,3-xylotriose and beta-1,3-xylotetraose. As hydrolysis proceeds an isomeric xylotriose is detectable as well as xylose, beta-1,3-xylobiose, beta-1,3-xylotriose and beta-1,3-xylotetraose Aspergillus terreus A-07 xylose + xylooligosaccharides
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.32 40
-
 enzyme form EF-4 and EF-5 Aspergillus terreus
3.2.1.32 45
-
 enzyme form EF-2 Aspergillus terreus
3.2.1.32 50
-
 enzyme form EF-3 and EF-6 Aspergillus terreus
3.2.1.32 55
-
 enzyme form EF-1 Aspergillus terreus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.32 50
-
 pH 4.5, 1 h, enzyme forms EF-5 and EF-6, stable up to Aspergillus terreus
3.2.1.32 60
-
 pH 4.5, 1 h, enzyme forms EF-1, EF-2, EF-3 and EF-4 are stable, enzyme form EF-5 and EF-6 rapidly lose activity Aspergillus terreus
3.2.1.32 70
-
 pH 4.5, 1 h, rapid decrease of activity of enzyme forms EF-1, EF-2, EF-3 and EF-4 Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.32 4
-
 enzyme form EF-4 Aspergillus terreus
3.2.1.32 4.5
-
 enzyme forms EF-5 and EF-6 Aspergillus terreus
3.2.1.32 5
-
 enzyme forms EF-1 and EF-3 Aspergillus terreus
3.2.1.32 5.5
-
 enzyme form EF-2 Aspergillus terreus