Any feedback?
Literature summary extracted from
- Extracellular isoamylase produced by the yeast Lipomyces kononenkoae (1982), Appl. Environ. Microbiol., 44, 1253-1257.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.68 | nutrition | production of high-maltose syrups and highly purified maltose by a combination of debranching enzymes | Lipomyces kononenkoae |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.68 | very unstable, even at low temperatures | Lipomyces kononenkoae |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.68 | extracellular | - |
Lipomyces kononenkoae | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.68 | 65000 | - |
gel filtration, 0.5-m Bio-Gel A | Lipomyces kononenkoae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.68 | starch + H2O | Lipomyces kononenkoae | - |
? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.68 | Lipomyces kononenkoae | - |
yeast, strain IGC 4051 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.68 | - |
Lipomyces kononenkoae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.68 | 52 | - |
- |
Lipomyces kononenkoae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.68 | amylopectin + H2O | or the beta-limit dextrins, cleaves the branching points completely in vitro | Lipomyces kononenkoae | maltose + maltooligosaccharides | - |
? | |
| 3.2.1.68 | D-glucose + H2O | 59% of the activity with dextrin as substrate | Lipomyces kononenkoae | ? | - |
? | |
| 3.2.1.68 | dextrin + H2O | enzyme activity 100% | Lipomyces kononenkoae | ? | - |
? | |
| 3.2.1.68 | glycogen + H2O | cleaves the branching points completely in vitro | Lipomyces kononenkoae | maltose + maltooligosaccharides | - |
? | |
| 3.2.1.68 | glycogen + H2O | or the beta-limit dextrins | Lipomyces kononenkoae | maltose + maltooligosaccharides | - |
? | |
| 3.2.1.68 | maltotriose + H2O | activity 28% | Lipomyces kononenkoae | ? | - |
? | |
| 3.2.1.68 | panose + H2O | acts very slowly, small amounts of product formed after 24 h | Lipomyces kononenkoae | D-glucose + maltose | - |
? | |
| 3.2.1.68 | saccharose + H2O | activity 67% | Lipomyces kononenkoae | ? | - |
? | |
| 3.2.1.68 | starch + H2O | - |
Lipomyces kononenkoae | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.68 | glycogen 6-glucanohydrolase | - |
Lipomyces kononenkoae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.68 | 30 | - |
starch | Lipomyces kononenkoae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.68 | 5.6 | - |
starch | Lipomyces kononenkoae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
