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Literature summary extracted from

  • Fukasawa, S.; Kobayashi, H.
    Properties of the agarase from a luminous bacterium, Vibrio harveyi (1987), Agric. Biol. Chem., 51, 269-270.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.81 NaCl at low concentrations, 1%, no effect between 3-8% Vibrio harveyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.81 21000
-
 SDS-PAGE Pseudomonas sp.
3.2.1.81 26000
-
-
 Flavobacterium flevense
3.2.1.81 32000
-
-
 Pseudoalteromonas atlantica
3.2.1.81 49000
-
 gel filtration Vibrio harveyi
3.2.1.81 50000
-
 SDS-PAGE Vibrio harveyi
3.2.1.81 63000
-
 SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.81 agarose + H2O Vibrio harveyi
-
 neoagarotetraose
-
 ir
3.2.1.81 agarose + H2O Pseudomonas sp.
-
 neoagarotetraose
-
 ir
3.2.1.81 agarose + H2O Pseudoalteromonas atlantica
-
 neoagarotetraose
-
 ir
3.2.1.81 agarose + H2O Flavobacterium flevense
-
 neoagarotetraose
-
 ir
3.2.1.81 agarose + H2O Pseudomonas sp. W7
-
 neoagarotetraose
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.81 Flavobacterium flevense
-
-
-
3.2.1.81 Pseudoalteromonas atlantica
-
-
-
3.2.1.81 Pseudomonas sp.
-
 W7
-
3.2.1.81 Pseudomonas sp. W7
-
 W7
-
3.2.1.81 Vibrio harveyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.81
-
 Vibrio harveyi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.81 22
-
-
 Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.81 agarose + H2O
-
 Vibrio harveyi neoagarotetraose
-
 ir
3.2.1.81 agarose + H2O
-
 Pseudomonas sp. neoagarotetraose
-
 ir
3.2.1.81 agarose + H2O
-
 Pseudoalteromonas atlantica neoagarotetraose
-
 ir
3.2.1.81 agarose + H2O
-
 Flavobacterium flevense neoagarotetraose
-
 ir
3.2.1.81 agarose + H2O
-
 Pseudomonas sp. W7 neoagarotetraose
-
 ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.81 40
-
 not stable above Vibrio harveyi
3.2.1.81 45
-
 about 50% of activity Vibrio harveyi
3.2.1.81 55
-
 about 25% of activity Vibrio harveyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.81 6
-
-
 Vibrio harveyi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.81 5 9 about 60% of activity Vibrio harveyi