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Literature summary extracted from

  • Lee, W.J.; Pyler, R.E.
    Nucleic acid degrading enzymes of barley malt. III. Adenosine nucleosidase from malted barley (1986), J. Am. Soc. Brew. Chem., 44, 86-90.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.2.7 labile during course of purification, addition of dithiothreitol required, 50% loss of activity if stored at -20°C in one week Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.7 Cu2+ marked inhibition at 1 mM Hordeum vulgare
3.2.2.7 HgCl2 strong inhibition at 1 mM Hordeum vulgare
3.2.2.7 p-chloromercuribenzoate 20% inhibition at 1 mM Hordeum vulgare
3.2.2.7 Zn2+ marked inhibition at 1 mM Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.7 0.27
-
 adenosine
-
 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.7 120000
-
 gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.7 2'-deoxyadenosine + H2O Hordeum vulgare hydrolyzes at 96% of the rate of adenosine 6-aminopurine + 2-deoxy-D-ribofuranose
-
 ?
3.2.2.7 2'-deoxyadenosine + H2O Hordeum vulgare 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside 6-aminopurine + 2-deoxy-D-ribofuranose
-
 ?
3.2.2.7 adenosine + H2O Hordeum vulgare 6-aminopurine-9-beta-D-ribofuranoside 6-aminopurine + D-ribofuranose
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.7 Hordeum vulgare
-
 barley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.7 to homogeneity, chromatography steps Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.7 seedling malt Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.7 3.2
-
-
 Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
3.2.2.7 4°C, 20 mM phosphate, pH 7.2, 10 mM 2-mercaptoethanol, 70% activity remains, 12 weeks Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.7 2'-deoxyadenosine + H2O hydrolyzes at 96% of the rate of adenosine Hordeum vulgare 6-aminopurine + 2-deoxy-D-ribofuranose
-
 ?
3.2.2.7 2'-deoxyadenosine + H2O 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside Hordeum vulgare 6-aminopurine + 2-deoxy-D-ribofuranose
-
 ?
3.2.2.7 adenosine + H2O 6-aminopurine-9-beta-D-ribofuranoside Hordeum vulgare 6-aminopurine + D-ribofuranose
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.7 60
-
-
 Hordeum vulgare

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.2.7 30 60
-
 Hordeum vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.7 60
-
 activity sharply decreases above Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.7 5
-
 same activity in Tris or phosphate buffer, no requirement for inorganic phosphate Hordeum vulgare