Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Burch, L.R.; Stuchbury, T.
    Purification and properties of adenosine nucleosidases from tomato (Lycopersicon esculentum) roots and leaves (1986), J. Plant Physiol., 125, 267-273.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.7 Inosine at 0.2 mM: 8.5% inhibition for form Lf, 10.5% inhibition for form R2, form R1 not inhibited Solanum lycopersicum
3.2.2.7 N6-Benzyladenosine 36.5% inhibition for form R1, 1.6% for form R2, form Lf not inhibited at 0.2 mM Solanum lycopersicum
3.2.2.7 N6-isopentenyladenosine inhibition between 28.6% and 36.7% for forms R1, R2 and Lf at 0.2 mM Solanum lycopersicum
3.2.2.7 zeatin riboside competitive inhibition between 32% and 25% for forms R1, R2 and Lf at 0.2 mM Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.7 0.006
-
 adenosine from Lf Solanum lycopersicum
3.2.2.7 0.009
-
 adenosine form R2 Solanum lycopersicum
3.2.2.7 0.025
-
 adenosine form R1 Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.7 68000
-
 gel filtration Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.7 adenosine + H2O Solanum lycopersicum component of the purine salvage pathway 6-aminopurine + D-ribofuranose
-
 ir
3.2.2.7 adenosine + H2O Solanum lycopersicum implicated in metabolism of cytokinins 6-aminopurine + D-ribofuranose
-
 ir
3.2.2.7 adenosine + H2O Solanum lycopersicum 6-aminopurine-9-beta-D-ribofuranoside 6-aminopurine + D-ribofuranose
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.7 Solanum lycopersicum
-
 three forms: R1, R2 and Lf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.7 form R2 to homogeneity, forms R1 and Lf partial Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.7 leaf form Lf Solanum lycopersicum
-
3.2.2.7 root two forms, R1 and R2 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.7 adenosine + H2O component of the purine salvage pathway Solanum lycopersicum 6-aminopurine + D-ribofuranose
-
 ir
3.2.2.7 adenosine + H2O implicated in metabolism of cytokinins Solanum lycopersicum 6-aminopurine + D-ribofuranose
-
 ir
3.2.2.7 adenosine + H2O 6-aminopurine-9-beta-D-ribofuranoside Solanum lycopersicum 6-aminopurine + D-ribofuranose
-
 ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.7 5
-
 for form R1 Solanum lycopersicum
3.2.2.7 6
-
 forms R2 and Lf Solanum lycopersicum