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Literature summary extracted from
- Tartaric acid metabolism. IX. Synthesis with tartrate epoxidase (1969), J. Biol. Chem., 244, 2078-2084.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.3.2.4 | - |
Pseudomonas putida |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.3.2.4 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Pseudomonas putida |  |
| 3.3.2.4 | p-chloromercuribenzoate | - |
Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.3.2.4 | 0.885 | - |
D,L-trans-2,3-epoxysuccinate | - |
Pseudomonas putida | |
| 3.3.2.4 | 2.15 | - |
L-trans-2,3-epoxysuccinate | - |
Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.4 | 66300 | - |
sedimentation velocity, diffusion coefficient | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.4 | trans-2,3-epoxysuccinate + H2O | Pseudomonas putida | carbon source | meso-tartrate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.3.2.4 | Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.3.2.4 | - |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.4 | 229 | - |
- |
Pseudomonas putida |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.3.2.4 | -15°C | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.4 | D-trans-2,3-epoxysuccinate + H2O | - |
Pseudomonas putida | meso-tartrate | - |
? | |
| 3.3.2.4 | L-trans-2,3-epoxysuccinate + H2O | - |
Pseudomonas putida | meso-tartrate | - |
? | |
| 3.3.2.4 | trans-2,3-epoxysuccinate + H2O | D- and L-trans-2,3-epoxysuccinate, not: cis-epoxysuccinate | Pseudomonas putida | meso-tartrate | - |
? | |
| 3.3.2.4 | trans-2,3-epoxysuccinate + H2O | carbon source | Pseudomonas putida | meso-tartrate | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.3.2.4 | 83.3 | - |
D-trans-2,3-epoxysuccinate | - |
Pseudomonas putida | |
| 3.3.2.4 | 317 | - |
L-trans-2,3-epoxysuccinate | - |
Pseudomonas putida | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.4 | 8.6 | 8.9 | - |
Pseudomonas putida |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.4 | 7 | 10 | pH 7: 10% of optimal activity, pH 10: 20% of optimal activity | Pseudomonas putida |
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