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Literature summary extracted from

  • te Broemmelstroet, B.W.; Hensgens, C.M.H.; Keltjens, J.T.; van der Drift, C.; Vogels, G.D.
    Purification and characterization of coenzyme F420-dependent 5,10-methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautothrophicum strain DELTAH (1991), Biochim. Biophys. Acta, 1073, 77-84.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.98.1 0.033
-
 5,10-methylenetetrahydromethanopterin
-
 Methanothermobacter thermautotrophicus
1.5.98.1 0.065
-
 coenzyme F420
-
 Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.98.1 Cs+ monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+ Methanothermobacter thermautotrophicus
1.5.98.1 K+ monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+ Methanothermobacter thermautotrophicus
1.5.98.1 Li+ monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+ Methanothermobacter thermautotrophicus
1.5.98.1 Na+ monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+ Methanothermobacter thermautotrophicus
1.5.98.1 Na+ 3 M NaCl stimulates 5fold Methanothermobacter thermautotrophicus
1.5.98.1 NH4+ monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+ Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.98.1 36000
-
 6 * 36000, SDS-PAGE Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
1.5.98.1 Methanothermobacter thermautotrophicus
-
-
-
1.5.98.1 Methanothermobacter thermautotrophicus DELTAH
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.98.1
-
 Methanothermobacter thermautotrophicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 + H+ = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 ternary complex mechanism Methanothermobacter thermautotrophicus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.98.1 736
-
-
 Methanothermobacter thermautotrophicus

Storage Stability

EC Number Storage Stability Organism
1.5.98.1 4°C, 0.5 mM 3-[(3-cholamidopropyl)dimethylammonio]1-propanesulfonate, stable for more than 6 months Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 r Methanothermobacter thermautotrophicus 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 absolutely dependent on coenzyme F420 Methanothermobacter thermautotrophicus 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 r Methanothermobacter thermautotrophicus DELTAH 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 absolutely dependent on coenzyme F420 Methanothermobacter thermautotrophicus DELTAH 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.98.1 hexamer 6 * 36000, SDS-PAGE Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.98.1 60
-
-
 Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.98.1 6
-
 oxidation of 5,10-methylenetetrahydromethanopterin Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.98.1 coenzyme F420 required Methanothermobacter thermautotrophicus