Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Klein, A.R.; Koch, J.; Stetter, K.O.; Thauer, R.K.
    Two N5,N10-methylenetetrahydromethanopterin dehydrogenases in the extreme thermophile Methanopyrus kandleri: characterization of the coenzyme F420-dependent enzyme (1993), Arch. Microbiol., 160, 186-192.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.98.1 0.02
-
 coenzyme F420
-
 Methanopyrus kandleri
1.5.98.1 0.08
-
 5,10-methylenetetrahydromethanopterin
-
 Methanopyrus kandleri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.98.1 (NH4)2SO4 very high salt concentration are required for high activity. Highest specific acitivity in presence of 2 M (NH4)2SO4. Other salts including NaH2PO4, KH2PO4 and NaCl are also effective in stimulating the enzyme activity Methanopyrus kandleri
1.5.98.1 K+ very high salt concentration is required for high activity. Highest specific acitivity in presence of 2 M (NH4)2SO4. Other salts including NaH2PO4, KH2PO4 and NaCl are also effective in stimulating the enzyme activity Methanopyrus kandleri
1.5.98.1 Na+ very high salt concentration is required for high activity. Highest specific acitivity in presence of 2 M (NH4)2SO4. Other salts including NaH2PO4, KH2PO4 and NaCl are also effective in stimulating the enzyme activity Methanopyrus kandleri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.98.1 36000
-
 8 * 36000, SDS-PAGE Methanopyrus kandleri
1.5.98.1 300000
-
 gel filtration Methanopyrus kandleri

Organism

EC Number Organism UniProt Comment Textmining
1.5.98.1 Methanopyrus kandleri
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.5.98.1 moderately stable against inactivation by air. 50% loss of activity within 12 h at 4°C under aerobic conditions Methanopyrus kandleri

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.98.1
-
 Methanopyrus kandleri

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 + H+ = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 ternary complex mechanism Methanopyrus kandleri
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.98.1 1320
-
-
 Methanopyrus kandleri

Storage Stability

EC Number Storage Stability Organism
1.5.98.1 -20°C, anaerobically stored, 50% loss of activity after several weeks Methanopyrus kandleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 absolutely dependent on coenzyme F420 Methanopyrus kandleri 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.98.1 octamer 8 * 36000, SDS-PAGE Methanopyrus kandleri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.98.1 75
-
-
 Methanopyrus kandleri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.98.1 90
-
 stable for 60 min in 50 mM potassium phosphate buffer, pH 6.8. Less than 50% loss of activity after 1 h in deionized water Methanopyrus kandleri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.98.1 4.5 6.5
-
 Methanopyrus kandleri

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.98.1 coenzyme F420 required Methanopyrus kandleri