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Literature summary extracted from

  • Rao, P.V.; Krishna, C.M.; Zigler, J.S.
    Identification and characterization of the enzymatic activity of zeta-Crystallin from guinea pig lens (1992), J. Biol. Chem., 267, 96-102.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.6.5.5 NADPH protects against inactivation caused by heat, NEM or H2O2 Cavia porcellus
1.6.5.5 very stable to freezing Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.5 5,5'-dithiobis(2-nitrobenzoate)
-
 Cavia porcellus
1.6.5.5 Cibacron blue 3GA
-
 Cavia porcellus
1.6.5.5 dicoumarol
-
 Cavia porcellus
1.6.5.5 H2O2
-
 Cavia porcellus
1.6.5.5 additional information no inactivation by 0.5 mM iodoacetate Cavia porcellus
1.6.5.5 NADP+
-
 Cavia porcellus
1.6.5.5 NEM
-
 Cavia porcellus
1.6.5.5 Nitrofurantoin
-
 Cavia porcellus
1.6.5.5 p-chloromercuriphenylsulfonate
-
 Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.5 0.005
-
 NADPH
-
 Cavia porcellus
1.6.5.5 0.01
-
 9,10-phenanthrenequinone
-
 Cavia porcellus
1.6.5.5 0.027
-
 5-hydroxy-1,4-naphthoquinone
-
 Cavia porcellus
1.6.5.5 0.143
-
 1,4-benzoquinone
-
 Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.5 Cavia porcellus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.6.5.5 NADPH + H+ + 2 quinone = NADP+ + 2 semiquinone enzyme acts through a one-electron transfer process Cavia porcellus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.5 lens
-
 Cavia porcellus
-

Storage Stability

EC Number Storage Stability Organism
1.6.5.5 -20°C, less than 10% loss of activity after 4-6 weeks Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.5 1,2-naphthoquinone + NADPH
-
 Cavia porcellus 1,2-naphthoquinol + NADP+
-
 ?
1.6.5.5 1,4-benzoquinone + NADPH 15.3% of the activity with 1,2-naphthoquinone Cavia porcellus 1,4-benzosemiquinone + NADP+
-
 ?
1.6.5.5 1,4-naphthoquinone + NADPH + H+ 2.6% of the activity with 1,2-naphthoquinone Cavia porcellus 1,4-naphthosemiquinone + NADP+
-
 ?
1.6.5.5 5-hydroxy-1,4-naphthoquinone + NADPH i.e. juglone, 12.5% of the activity with 1,2-naphthoquinone Cavia porcellus 5-hydroxy-1,4-naphthosemiquinone + NADP+
-
 ?
1.6.5.5 5-hydroxy-2-methyl-1,4-naphthoquinone + NADPH i.e. plumbagin, 0.9% of the activity with 1,2-naphthoquinone Cavia porcellus 5-hydroxy-2-methyl-1,4-naphthoquinol + NADP+
-
 ?
1.6.5.5 9,10-phenanthrenequinone + NADPH 50% of the activity with 1,2-naphthoquinone Cavia porcellus 9,10-phenanthrenesemiquinone + NADP+
-
 ?
1.6.5.5 methyl-1,4-benzoquinone + NADPH 20.6% of the activity with 1,2-naphthoquinone Cavia porcellus methyl-1,4-benzoquinol + NADP+
-
 ?
1.6.5.5 additional information inactive with: menadione, ubiquinone, 9,10-anthraquinone, vitamin K1, vitamin K2 Cavia porcellus ?
-
 ?
1.6.5.5 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol 3.8% of the activity with 1,2-naphthoquinone Cavia porcellus NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.5.5 45
-
 10 min, 65% loss of activity Cavia porcellus
1.6.5.5 51
-
 10 min, complete loss of activity Cavia porcellus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.5.5 2.6
-
 5-hydroxy-1,4-naphthoquinone
-
 Cavia porcellus
1.6.5.5 3.4
-
 1,4-benzoquinone
-
 Cavia porcellus
1.6.5.5 10
-
 9,10-phenanthrenequinone
-
 Cavia porcellus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.5 NADPH specific for Cavia porcellus