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Literature summary extracted from

  • R๖del, W.; Plaga, W.; Frank, W.; Knappe, J.
    Primary structures of Escherichia coli pyruvate formate-lyase and pyruvate-formate-lyase-activating enzyme deduced from the DNA nucleotide sequences (1988), Eur. J. Biochem., 177, 153-158.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.97.1.4
-
 Escherichia coli
2.3.1.54 structural gene pfl cloned and sequenced, homologous expression and overproduction in Escherichia coli K12 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.97.1.4 28035
-
 x * 28035, calculation from nucleotide sequence Escherichia coli
2.3.1.54 85000
-
 2 * 85000, homodimer, SDS-PAGE Escherichia coli
2.3.1.54 85120
-
 amino acid sequence calculation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.54 pyruvate + CoA Escherichia coli key enzyme of the glucose fermentation route in anaerobically growing cells acetyl-CoA + formate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.97.1.4 Escherichia coli
-
-
-
2.3.1.54 Escherichia coli
-
 K12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.54
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.97.1.4 S-adenosyl-L-methionine + dihydroflavodoxin + formate acetyltransferase-glycine
-
 Escherichia coli 5'-deoxyadenosine + methionine + flavodoxin + formate acetyltransferase-glycine-2-yl-radical
-
 ?
2.3.1.54 acetyl-CoA + formate
-
 Escherichia coli pyruvate + CoA
-
 r
2.3.1.54 pyruvate + CoA
-
 Escherichia coli acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + CoA key enzyme of the glucose fermentation route in anaerobically growing cells Escherichia coli acetyl-CoA + formate
-
 r

Subunits

EC Number Subunits Comment Organism
1.97.1.4 ? x * 28035, calculation from nucleotide sequence Escherichia coli
2.3.1.54 dimer 2 * 85000, homodimer, SDS-PAGE Escherichia coli