Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Buttlaire, D.H.
    Purification and properties of formyltetrahydrofolate synthetase (1980), Methods Enzymol., 66, 585-599.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.3
-
 Clostridium cylindrosporum

General Stability

EC Number General Stability Organism
6.3.4.3 purified enzyme as crystalline suspension, 15% loss of activity after 1 month Clostridium cylindrosporum
6.3.4.3 tetramer is stabilized by 0.1 M sulfate in absence of an active monovalent cation Clostridium cylindrosporum
6.3.4.3 unstable in absence of monovalent cations Clostridium cylindrosporum
6.3.4.3 unstable in presence of urea and guanidinium chloride Clostridium cylindrosporum

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.3 5,5'-dithiobis(2-nitrobenzoate)
-
 Clostridium cylindrosporum
6.3.4.3 Adenylyl imidodiphosphate competitive to ATP Clostridium cylindrosporum
6.3.4.3 ADP competitive to ATP Clostridium cylindrosporum
6.3.4.3 alpha,beta-methyleneadenosine 5'-triphosphate competitive to ATP Clostridium cylindrosporum
6.3.4.3 beta,gamma-methyleneadenosine 5'-triphosphate competitive to ATP Clostridium cylindrosporum
6.3.4.3 Formyltetrahydrofolate
-
 Clostridium cylindrosporum
6.3.4.3 Mersalyl
-
 Clostridium cylindrosporum
6.3.4.3 NEM
-
 Clostridium cylindrosporum
6.3.4.3 p-hydroxymercuribenzoate
-
 Moorella thermoacetica
6.3.4.3 phosphate
-
 Clostridium cylindrosporum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.3 additional information
-
 additional information
-
 Oryctolagus cuniculus
6.3.4.3 additional information
-
 additional information
-
 Moorella thermoacetica
6.3.4.3 additional information
-
 additional information monovalent cations decrease the Km for formate Clostridium cylindrosporum
6.3.4.3 0.025
-
 5,6,7,8-tetrahydropteroyltriglutamate
-
 Pisum sativum
6.3.4.3 0.025
-
 5,6,7,8-tetrahydropteroyltriglutamate
-
 Clostridium cylindrosporum
6.3.4.3 0.12
-
 ATP
-
 Saccharomyces cerevisiae
6.3.4.3 0.12
-
 ATP N10-formyltetrahydropteroyl-Glu3 Clostridium cylindrosporum
6.3.4.3 0.13
-
 ADP formyltetrahydrofolate synthesis Clostridium cylindrosporum
6.3.4.3 0.14
-
 ATP
-
 Pisum sativum
6.3.4.3 0.14
-
 ATP
-
 Clostridium cylindrosporum
6.3.4.3 0.22
-
 ATP
-
 Clostridium cylindrosporum
6.3.4.3 5
-
 phosphate
-
 Clostridium cylindrosporum
6.3.4.3 6.7
-
 formate wild-type enzyme Clostridium cylindrosporum
6.3.4.3 10
-
 N10-formyltetrahydrofolate
-
 Clostridium cylindrosporum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.3 Ca2+ the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Spinacia oleracea
6.3.4.3 Ca2+ the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Clostridium cylindrosporum
6.3.4.3 Cs+
-
 Spinacia oleracea
6.3.4.3 Cs+ specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ Clostridium cylindrosporum
6.3.4.3 divalent metal ion the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Spinacia oleracea
6.3.4.3 divalent metal ion the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Clostridium cylindrosporum
6.3.4.3 K+
-
 Saccharomyces cerevisiae
6.3.4.3 K+
-
 Oryctolagus cuniculus
6.3.4.3 K+
-
 Spinacia oleracea
6.3.4.3 K+
-
 Moorella thermoacetica
6.3.4.3 K+ specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ Clostridium cylindrosporum
6.3.4.3 Li+ specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ Clostridium cylindrosporum
6.3.4.3 Mg2+
-
 Saccharomyces cerevisiae
6.3.4.3 Mg2+
-
 Pisum sativum
6.3.4.3 Mg2+ the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Spinacia oleracea
6.3.4.3 Mg2+ the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Clostridium cylindrosporum
6.3.4.3 Mn2+ the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Spinacia oleracea
6.3.4.3 Mn2+ the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Clostridium cylindrosporum
6.3.4.3 monovalent cations
-
 Spinacia oleracea
6.3.4.3 monovalent cations specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ Clostridium cylindrosporum
6.3.4.3 Na+
-
 Spinacia oleracea
6.3.4.3 Na+ specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ Clostridium cylindrosporum
6.3.4.3 NH4+
-
 Oryctolagus cuniculus
6.3.4.3 NH4+
-
 Spinacia oleracea
6.3.4.3 NH4+
-
 Pisum sativum
6.3.4.3 NH4+
-
 Moorella thermoacetica
6.3.4.3 NH4+ specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ Clostridium cylindrosporum
6.3.4.3 Rb+
-
 Spinacia oleracea
6.3.4.3 Rb+ specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ Clostridium cylindrosporum
6.3.4.3 Tl+ specific monovalent cations required for maximal activity, order of effectiveness: NH4+, Tl+, Rb+ ~ K+, Cs+, Na+ ~ Li+ Clostridium cylindrosporum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.3 240000
-
 gel filtration, sedimentation analysis Clostridium cylindrosporum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate Gallus gallus two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Escherichia coli two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Homo sapiens two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Saccharomyces cerevisiae two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Oryctolagus cuniculus two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Neurospora crassa two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Ovis aries two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Spinacia oleracea two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Pisum sativum two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Priestia megaterium two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Pigeon two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Proteus vulgaris two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Clostridium cylindrosporum two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Clostridium acidi-urici two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Moorella thermoacetica two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Micrococcus aerogenes two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Veillonella parvula two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.3 Clostridium acidi-urici
-
-
-
6.3.4.3 Clostridium cylindrosporum
-
-
-
6.3.4.3 Escherichia coli
-
-
-
6.3.4.3 Gallus gallus
-
-
-
6.3.4.3 Homo sapiens
-
 trifunctional enzyme with 10-formyltetrahydrofolate synthetase, EC 6.3.4.3, 5,10-methenyltetrahydrofolate cyclohydrolase, EC 3.5.4.9, and 5,10-methylenetetrahydrofolate dehydrogenase activity, EC 1.5.1.5
-
6.3.4.3 Micrococcus aerogenes
-
-
-
6.3.4.3 Moorella thermoacetica
-
-
-
6.3.4.3 Neurospora crassa
-
-
-
6.3.4.3 Oryctolagus cuniculus
-
-
-
6.3.4.3 Ovis aries
-
 trifunctional enzyme with 10-formyltetrahydrofolate synthetase, EC 6.3.4.3, 5,10-methenyltetrahydrofolate cyclohydrolase, EC 3.5.4.9, and 5,10-methylenetetrahydrofolate dehydrogenase activity, EC 1.5.1.5
-
6.3.4.3 Pigeon
-
-
-
6.3.4.3 Pisum sativum
-
-
-
6.3.4.3 Priestia megaterium
-
-
-
6.3.4.3 Proteus vulgaris
-
-
-
6.3.4.3 Saccharomyces cerevisiae
-
 trifunctional enzyme with 10-formyltetrahydrofolate synthetase, EC 6.3.4.3, 5,10-methenyltetrahydrofolate cyclohydrolase, EC 3.5.4.9, and 5,10-methylenetetrahydrofolate dehydrogenase activity, EC 1.5.1.5
-
6.3.4.3 Spinacia oleracea
-
 spinach
-
6.3.4.3 Veillonella parvula
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.3
-
 Clostridium cylindrosporum

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.3 ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate substrate binds to the enzyme in a random fashion, products are not released until all substrates are bound Clostridium cylindrosporum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.3 leaf
-
 Spinacia oleracea
-
6.3.4.3 leaf
-
 Pisum sativum
-
6.3.4.3 leukocyte
-
 Homo sapiens
-
6.3.4.3 liver
-
 Gallus gallus
-
6.3.4.3 liver
-
 Oryctolagus cuniculus
-
6.3.4.3 liver
-
 Ovis aries
-
6.3.4.3 liver
-
 Pigeon
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.3 additional information
-
-
 Oryctolagus cuniculus
6.3.4.3 additional information
-
-
 Pisum sativum
6.3.4.3 additional information
-
-
 Clostridium cylindrosporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.3 ATP + formate + 5,6,7,8-tetrahydropteroyltriglutamate
-
 Pisum sativum ADP + phosphate + 10-formyltetrahydrofolyltriglutamate
-
 ?
6.3.4.3 ATP + formate + 5,6,7,8-tetrahydropteroyltriglutamate
-
 Clostridium cylindrosporum ADP + phosphate + 10-formyltetrahydrofolyltriglutamate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Gallus gallus ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Escherichia coli ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Homo sapiens ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Saccharomyces cerevisiae ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Oryctolagus cuniculus ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Neurospora crassa ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Ovis aries ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Spinacia oleracea ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Pisum sativum ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Priestia megaterium ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Pigeon ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Proteus vulgaris ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Clostridium acidi-urici ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Micrococcus aerogenes ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Veillonella parvula ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate r Clostridium cylindrosporum ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate r Moorella thermoacetica ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate ATP in form of MgATP2- Saccharomyces cerevisiae ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate ATP in form of MgATP2- Pisum sativum ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate ATP in form of MgATP2- Clostridium cylindrosporum ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Gallus gallus ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Escherichia coli ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Homo sapiens ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Saccharomyces cerevisiae ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Oryctolagus cuniculus ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Neurospora crassa ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Ovis aries ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Spinacia oleracea ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Pisum sativum ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Priestia megaterium ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Pigeon ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Proteus vulgaris ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Clostridium cylindrosporum ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Clostridium acidi-urici ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Moorella thermoacetica ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Micrococcus aerogenes ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate two different physiological roles: 1. Functions anabolically in most organisms to activate formate via the forward reaction, and brings it into the one-carbon metabolic pool as N10-formyltetrahydrofolate, 2. In purine-fermenting bacteria the enzyme probably functions catabolically in the terminal step of the purine degradative pathway. This reaction may be responsible for ATP production in these organisms Veillonella parvula ?
-
 ?
6.3.4.3 ATP + formate + tetrahydropteroyl-(Glu)n
-
 Oryctolagus cuniculus ADP + phosphate + 10-formyltetrahydropteroyl-(Glu)n
-
 ?
6.3.4.3 ATP + formate + tetrahydropteroyl-(Glu)n
-
 Pisum sativum ADP + phosphate + 10-formyltetrahydropteroyl-(Glu)n
-
 ?
6.3.4.3 ATP + formate + tetrahydropteroyl-(Glu)n r, n: 3 Clostridium cylindrosporum ADP + phosphate + 10-formyltetrahydropteroyl-(Glu)n
-
 ?
6.3.4.3 Carbamoyl phosphate + ADP
-
 Clostridium cylindrosporum ? + ATP
-
 ?
6.3.4.3 dATP + formate + tetrahydrofolate 37% of the activity relative to ATP Clostridium cylindrosporum dADP + phosphate + 10-formyltetrahydrofolate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.3 42
-
-
 Clostridium cylindrosporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.3 7 9
-
 Clostridium cylindrosporum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.4.3 6.5 9 unstable below pH 6.5 and above pH 9.0 Clostridium cylindrosporum