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Literature summary extracted from

  • Matsuda, Y.; Shimura, K.; Shiraki, H.; Nakagawa, H.
    Purification and properties of adenylosuccinate synthetase from Yoshida sarcoma ascites tumor cells (1980), Biochim. Biophys. Acta, 616, 340-350.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.4 ADP
-
 Rattus norvegicus
6.3.4.4 AMP
-
 Rattus norvegicus
6.3.4.4 Ca2+ in presence of Mg2+ Rattus norvegicus
6.3.4.4 Cd2+ in presence of Mg2+ Rattus norvegicus
6.3.4.4 Cu2+ in presence of Mg2+ Rattus norvegicus
6.3.4.4 dGTP
-
 Rattus norvegicus
6.3.4.4 GDP
-
 Rattus norvegicus
6.3.4.4 GMP
-
 Rattus norvegicus
6.3.4.4 guanosine
-
 Rattus norvegicus
6.3.4.4 Hadacidin
-
 Rattus norvegicus
6.3.4.4 Hg2+ reversed by addition of DTT Rattus norvegicus
6.3.4.4 IDP
-
 Rattus norvegicus
6.3.4.4 Mn2+ in presence of Mg2+ Rattus norvegicus
6.3.4.4 Pb2+ in presence of Mg2+ Rattus norvegicus
6.3.4.4 PCMB
-
 Rattus norvegicus
6.3.4.4 XMP
-
 Rattus norvegicus
6.3.4.4 Zn2+ in presence of Mg2+ Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.4 0.07
-
 GTP
-
 Rattus norvegicus
6.3.4.4 0.41
-
 IMP
-
 Rattus norvegicus
6.3.4.4 0.98
-
 L-Asp
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.4 Ca2+ 14.1% of the activation relative to Mg2+ Rattus norvegicus
6.3.4.4 Co2+ 34.8% of the activation relative to Mg2+ Rattus norvegicus
6.3.4.4 Cu2+ 10.9% of the activation relative to Mg2+ Rattus norvegicus
6.3.4.4 Mg2+ full activity in presence of Mg2+ Rattus norvegicus
6.3.4.4 Mn2+ 37% of the activation relative to Mg2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.4 47000
-
 2 * 47000, sedimentation equilibrium analysis in presence of 6 M guanidine-HCl Rattus norvegicus
6.3.4.4 102000
-
 Yoshida sarcoma ascites tumor cells, sedimentation equilibrium analysis Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.4
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.4 Yoshida ascites sarcoma cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.4 2.36
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
6.3.4.4 -20°C, or 0°C, rapid loss of enzyme activity in dilute solution Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.4 GTP + IMP + L-Asp
-
 Rattus norvegicus GDP + phosphate + adenylosuccinate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.4 dimer 2 * 47000, sedimentation equilibrium analysis in presence of 6 M guanidine-HCl Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.4 6.8 7
-
 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.4.4 7
-
 most stable at pH 7.0 in phosphate buffer Rattus norvegicus