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Literature summary extracted from

  • Markham, G.D.; Reed, G.H.
    Adenylosuccinate synthetase from Azotobacter vinelandii: purification, properties and steady-state kinetics (1977), Arch. Biochem. Biophys., 184, 24-35.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.4 2-mercaptosuccinate
-
 Azotobacter vinelandii
6.3.4.4 AMP
-
 Azotobacter vinelandii
6.3.4.4 Cl-
-
 Azotobacter vinelandii
6.3.4.4 Cl3CCO2-
-
 Azotobacter vinelandii
6.3.4.4 fumarate
-
 Azotobacter vinelandii
6.3.4.4 HCO2-
-
 Azotobacter vinelandii
6.3.4.4 I-
-
 Azotobacter vinelandii
6.3.4.4 Maleate
-
 Azotobacter vinelandii
6.3.4.4 NO2-
-
 Azotobacter vinelandii
6.3.4.4 NO3-
-
 Azotobacter vinelandii
6.3.4.4 oxaloacetate
-
 Azotobacter vinelandii
6.3.4.4 phosphate
-
 Azotobacter vinelandii
6.3.4.4 SCN- i.e. thiocyanate Azotobacter vinelandii
6.3.4.4 SO32-
-
 Azotobacter vinelandii
6.3.4.4 succinate
-
 Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.4 54000
-
 2 * 54000, SDS-PAGE Azotobacter vinelandii
6.3.4.4 110000
-
 gel filtration Azotobacter vinelandii

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.4 Azotobacter vinelandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.4
-
 Azotobacter vinelandii

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.4 GTP + IMP + L-aspartate = GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP sequential mechanism with a fully random order of substrate addition Azotobacter vinelandii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.4 GTP + IMP + L-Asp r Azotobacter vinelandii GDP + phosphate + adenylosuccinate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.4 dimer 2 * 54000, SDS-PAGE Azotobacter vinelandii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.4 6.5 7.4
-
 Azotobacter vinelandii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.4.4 6 8 50% of maximal activity at pH 6.0 and 8.0 Azotobacter vinelandii