Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hongo, S.; Matsumoto, T.; Sato, T.
    Purification and properties of asparagine synthetase from rat liver (1978), Biochim. Biophys. Acta, 522, 258-266.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.4 ADP weak Rattus norvegicus
6.3.5.4 AMP weak Rattus norvegicus
6.3.5.4 Asn
-
 Rattus norvegicus
6.3.5.4 Carbamoyl phosphate
-
 Rattus norvegicus
6.3.5.4 CMP weak Rattus norvegicus
6.3.5.4 CTP
-
 Rattus norvegicus
6.3.5.4 diphosphate
-
 Rattus norvegicus
6.3.5.4 GDP weak Rattus norvegicus
6.3.5.4 GMP weak Rattus norvegicus
6.3.5.4 GTP
-
 Rattus norvegicus
6.3.5.4 IMP weak Rattus norvegicus
6.3.5.4 iodoacetamide
-
 Rattus norvegicus
6.3.5.4 ITP
-
 Rattus norvegicus
6.3.5.4 NEM
-
 Rattus norvegicus
6.3.5.4 nucleoside triphosphates except ATP Rattus norvegicus
6.3.5.4 p-chloromercuribenzoate
-
 Rattus norvegicus
6.3.5.4 sulfhydryl reagents
-
 Rattus norvegicus
6.3.5.4 UMP weak Rattus norvegicus
6.3.5.4 UTP
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.4 0.14
-
 ATP
-
 Rattus norvegicus
6.3.5.4 0.76
-
 L-Asp
-
 Rattus norvegicus
6.3.5.4 4.3
-
 L-Gln
-
 Rattus norvegicus
6.3.5.4 10
-
 NH4+
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.4 Cl- required, Km: 1.7 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.4 110000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.4
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.4 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-Asp + L-Gln
-
 Rattus norvegicus AMP + diphosphate + Asn + Glu
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.5.4 additional information
-
 a combination of L-Asp, ATP, and Mg2+ protects against heat inactivation Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.4 7.4 7.6 Gln-dependent activity Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.5.4 8
-
 most stable around pH 8 at 4°C Rattus norvegicus