Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Springate, C.F.; Stachow, C.S.
    Fructose-bisphosphatase from Rhodopseudomonas palustris (1982), Methods Enzymol., 90, 378-384.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.11 2-mercaptoethanol 1 mM, stimulates Rhodopseudomonas palustris
3.1.3.11 Cys 1 mM, stimulates Rhodopseudomonas palustris
3.1.3.11 dithiothreitol 1 mM stimulates Rhodopseudomonas palustris
3.1.3.11 EDTA
-
 Rhodopseudomonas palustris
3.1.3.11 sulfhydryl reagents 1 mM, stimulate in order of decreasing stimulation activity: glutathione, cysteine, dithiothreitol, 2-mercaptoethanol Rhodopseudomonas palustris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 AMP no inhibition Rhodopseudomonas palustris
3.1.3.11 p-hydroxymercuribenzoate strong Rhodopseudomonas palustris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.03
-
 sedoheptulose 1,7-diphosphate
-
 Rhodopseudomonas palustris
3.1.3.11 0.045
-
 fructose 1,6-diphosphate
-
 Rhodopseudomonas palustris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Mg2+ Km: 3.3 mM Rhodopseudomonas palustris
3.1.3.11 Mg2+ divalent cation required. Mn2+ or Mg2+ Rhodopseudomonas palustris
3.1.3.11 Mn2+ divalent cation required. Mn2+ or Mg2+ Rhodopseudomonas palustris
3.1.3.11 Mn2+ Km: 0.078 mM Rhodopseudomonas palustris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 130000
-
 sucrose density gradient centrifugation Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.11 D-fructose 1,6-diphosphate + H2O Rhodopseudomonas palustris the enzyme may be involved in the regulation of the Calvin-Bassham carbon reduction cycle ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11
-
 Rhodopseudomonas palustris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.11 leaf
-
 Rhodopseudomonas palustris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.11 additional information
-
-
 Rhodopseudomonas palustris

Storage Stability

EC Number Storage Stability Organism
3.1.3.11 -20°C, 0.1 mM MnCl2, 1 mM DTT, 0.01 M Tris-HCl buffer, pH 7.4, stable for at least 3 months Rhodopseudomonas palustris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-diphosphate + H2O the enzyme may be involved in the regulation of the Calvin-Bassham carbon reduction cycle Rhodopseudomonas palustris ?
-
 ?
3.1.3.11 D-fructose 1,6-diphosphate + H2O
-
 Rhodopseudomonas palustris D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 sedoheptulose 1,7-bisphosphate + H2O at 22.4% of the activity with fructose 1,6-diphosphate Rhodopseudomonas palustris sedoheptulose 7-phosphate + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.11 8.5
-
-
 Rhodopseudomonas palustris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.11 7.5 8.5 pH 7.5: 65% of maximal activity, pH 8.5: optimum Rhodopseudomonas palustris