Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Huang, Y.Z.; Knox, W.E.
    Glutamine-dependent asparagine synthetase in fetal, adult and neoplastic rat tissues (1975), Enzyme, 19, 314-328.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.4 additional information enzyme from fetal liver extracts is significantly inhibited when combined with adult liver or tumor extracts Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.4 0.31
-
 L-Asp
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.4 Mg2+ divalent metal ion required, Mg2+ more effective than Mn2+ Rattus norvegicus
6.3.5.4 Mn2+ 10% of the activity relative to Mg2+ Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.4 brain low Rattus norvegicus
-
6.3.5.4 breast cancer cell line carcinoma Rattus norvegicus
-
6.3.5.4 kidney
-
 Rattus norvegicus
-
6.3.5.4 liver high activity in fetal liver and low activity in adult liver Rattus norvegicus
-
6.3.5.4 LLC-WRC 256 cell
-
 Rattus norvegicus
-
6.3.5.4 lung
-
 Rattus norvegicus
-
6.3.5.4 McA-RH7777 cell
-
 Rattus norvegicus
-
6.3.5.4 ovary
-
 Rattus norvegicus
-
6.3.5.4 pancreas adult pancreas is the most active tissue found Rattus norvegicus
-
6.3.5.4 small intestine low Rattus norvegicus
-
6.3.5.4 spleen low Rattus norvegicus
-
6.3.5.4 testis
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-Asp + L-Gln
-
 Rattus norvegicus AMP + diphosphate + Asn + Glu
-
 ?
6.3.5.4 ATP + L-Asp + NH3 85% of the activity relative to Gln Rattus norvegicus AMP + diphosphate + Asn
-
 ?
6.3.5.4 additional information ATP-diphosphate exchange reaction Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.4 7.5 8
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.5.4 6.5 8.5 minor variation of activity in this pH range Rattus norvegicus