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Literature summary extracted from

  • Liu, W.; Madsen, N.B.; Fan, B.; Zucker, K.A.; Glew, R.H.; Fry, D.E.
    Effects of oligosaccharide binding on glycogen debranching enzyme activity and conformation (1995), Biochemistry, 34, 7056-7061.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.33 alpha-cyclodextrin 20 mM: 2.3fold activation Oryctolagus cuniculus
3.2.1.33 amylopectin potent activator Oryctolagus cuniculus
3.2.1.33 glucooligosaccharides with four or more glucose units, uncompetitive activation Oryctolagus cuniculus
3.2.1.33 glycogen potent activator Oryctolagus cuniculus
3.2.1.33 maltohexaose 20 mM: 1.86fold activation Oryctolagus cuniculus
3.2.1.33 maltopentaose 20 mM: 1.45fold activation Oryctolagus cuniculus
3.2.1.33 maltotetraose 20 mM: 1.15fold activation Oryctolagus cuniculus
3.2.1.33 additional information mechanism of activation Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.33 D-glucose competitive Oryctolagus cuniculus
3.2.1.33 glucooligosaccharides containing one, two, or three glucose, competitive inhibitors Oryctolagus cuniculus
3.2.1.33 maltose competitive Oryctolagus cuniculus
3.2.1.33 maltotriose competitive Oryctolagus cuniculus
3.2.1.33 additional information inhibition mechanism Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.33 additional information
-
 additional information kinetic data Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O Oryctolagus cuniculus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.33 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.33 glycogen debranching enzyme Oryctolagus cuniculus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.33 6-O-alpha-D-glucosyl cyclomaltoheptaose + H2O = D-glucose + cyclomaltoheptaose mechanism Oryctolagus cuniculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.33 muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.33 11
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.33 alpha-D-glucosyl fluoride
-
 Oryctolagus cuniculus fluoride + D-glucose
-
 ?
3.2.1.33 glycogen + H2O slowly hydrolyzed Oryctolagus cuniculus glycogen + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O
-
 Oryctolagus cuniculus limit dextrin + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O
-
 Oryctolagus cuniculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.33 glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Oryctolagus cuniculus