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Literature summary extracted from

  • Patterson jr., M.K.; Orr, G.R.
    Asparagine biosynthesis by the Novikoff hepatoma. Isolation, purification, properties, and mechanism studies of the enzyme system (1968), J. Biol. Chem., 243, 376-380.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.4 azaserine
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.4 1.1
-
 L-Gln
-
 Rattus norvegicus
6.3.5.4 120
-
 NH4+
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.4 Mg2+ divalent metal ion required, Mg2+ more effective than Mn2+ Rattus norvegicus
6.3.5.4 Mg2+ Km: 23.7 Rattus norvegicus
6.3.5.4 Mn2+ at 2 mM 22.0% of the activity relative to Mg2+ Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.4
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.4 Novikoff hepatoma cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.4 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-Asp + L-Gln
-
 Rattus norvegicus AMP + diphosphate + Asn + Glu
-
 ?
6.3.5.4 ATP + L-Asp + NH2OH
-
 Rattus norvegicus AMP + diphosphate + beta-aspartylhydroxamate
-
 ?
6.3.5.4 GTP + L-Asp + L-Gln 5.6% of the activity relative to ATP Rattus norvegicus GMP + diphosphate + Asn + Glu
-
 ?
6.3.5.4 additional information the synthesis of Asn in mammalian tissues proceeds through the intermediate beta-aspartyladenylate Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.4 6.6 8
-
 Rattus norvegicus