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Literature summary extracted from

  • Nakakuki, T.; Azuma, K.; Monma, M.; Kainuma, K.
    Purification and some properties of an extracellular exo-maltohexaohydrolase from an Aerobacter aerogenes mutant (1982), J. Jpn. Soc. Starch Sci., 29, 188-197.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.98 Ca2+ stabilizes, enzyme is more thermostable in the presence of Ca2+ Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.98 Ag+ extracellular enzyme, strong inhibition Klebsiella pneumoniae
3.2.1.98 AgNO3 extracellular enzyme, strong inhibition Klebsiella pneumoniae
3.2.1.98 Cu2+ extracellular enzyme, strong inhibition Klebsiella pneumoniae
3.2.1.98 CuSO4 extracellular enzyme, strong inhibition Klebsiella pneumoniae
3.2.1.98 FeSO4 extracellular enzyme Klebsiella pneumoniae
3.2.1.98 Hg2+ extracellular enzyme, completely Klebsiella pneumoniae
3.2.1.98 HgCl2 extracellular enzyme, completely Klebsiella pneumoniae
3.2.1.98 iodoacetamide extracellular enzyme, 1 mM: 11% inactivation; slight inactivation Klebsiella pneumoniae
3.2.1.98 p-chloromercuribenzoate extracellular enzyme, 1 mM: 46% inactivation Klebsiella pneumoniae
3.2.1.98 Zn2+ extracellular enzyme, strong inhibition Klebsiella pneumoniae
3.2.1.98 ZnSO4 extracellular enzyme, strong inhibition Klebsiella pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.98 cell bound
-
 Klebsiella pneumoniae
-
-
3.2.1.98 extracellular ultraviolet induced mutant Klebsiella pneumoniae
-
-
3.2.1.98 extracellular extracellular enzyme has the same characteristics as the cell-bound enzyme: enzyme is secreted extracellulary due to mutation of parent strain of Klebsiella pneumoniae Klebsiella pneumoniae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.98 Ca2+ native enzyme, activation Klebsiella pneumoniae
3.2.1.98 Ca2+ extracellular enzyme, slight activation Klebsiella pneumoniae
3.2.1.98 CaCl2 extracellular enzyme, slight activation Klebsiella pneumoniae
3.2.1.98 Co2+ extracellular enzyme, slight activation Klebsiella pneumoniae
3.2.1.98 CoCl2 extracellular enzyme, slight activation Klebsiella pneumoniae
3.2.1.98 Mn2+ extracellular enzyme, slight activation Klebsiella pneumoniae
3.2.1.98 MnSO4 extracellular enzyme, slight activation Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.98 48000
-
 cell-bound and extracellular enzyme, gel filtration Klebsiella pneumoniae
3.2.1.98 65000
-
 extracellular enzyme, SDS-PAGE Klebsiella pneumoniae
3.2.1.98 67000
-
 cell-bound enzyme, SDS-PAGE Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.98 Klebsiella pneumoniae
-
 Aerobacter aerogenes
-
3.2.1.98 Klebsiella pneumoniae
-
 ultraviolet-induced mutant
-
3.2.1.98 Klebsiella pneumoniae
-
 ultraviolet-induced mutant from Aerobacter aerogenes IFO-3321
-
3.2.1.98 Klebsiella pneumoniae
-
 IFO-3321
-
3.2.1.98 Klebsiella pneumoniae IFO-3321
-
 IFO-3321
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.98
-
 Klebsiella pneumoniae

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.98 (Glcalpha(1-4))m + H2O = (Glcalpha(1-4))m-6 + maltohexaose reaction mechanism is exo Klebsiella pneumoniae
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.98 culture filtrate
-
 Klebsiella pneumoniae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.98 8.2
-
 cell-bound enzyme Klebsiella pneumoniae
3.2.1.98 18.4
-
 extracellular enzyme Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.98 amylopectin + H2O
-
 Klebsiella pneumoniae maltohexaose + ?
-
 ?
3.2.1.98 amylopectin beta-amylase limit dextrin + H2O
-
 Klebsiella pneumoniae oligosaccharide branched oligosaccharides containing 5 to 10 glucose units, B5-B10 ?
3.2.1.98 amylose + H2O short-chain amylose, DP, average degree of polymerization, = 23 Klebsiella pneumoniae maltohexaose
-
 ?
3.2.1.98 amylose + H2O reduced short-chain amylose: about 95% of the rate of short-chain amylose Klebsiella pneumoniae maltohexaose
-
 ?
3.2.1.98 amylose + H2O potato amylose: about 57% of the rate of soluble starch Klebsiella pneumoniae maltohexaose
-
 ?
3.2.1.98 amylose + H2O short-chain amylose, DP, average degree of polymerization, = 23 Klebsiella pneumoniae IFO-3321 maltohexaose
-
 ?
3.2.1.98 amylose + H2O reduced short-chain amylose: about 95% of the rate of short-chain amylose Klebsiella pneumoniae IFO-3321 maltohexaose
-
 ?
3.2.1.98 glycogen + H2O
-
 Klebsiella pneumoniae maltohexaose
-
 ?
3.2.1.98 glycogen beta-amylase limit dextrin + H2O
-
 Klebsiella pneumoniae oligosaccharide branched oligosaccharides containing 5 to 10 glucose units, B5-B10 ?
3.2.1.98 maltohexaose + H2O poor substrate Klebsiella pneumoniae maltose + maltotetraose main product ?
3.2.1.98 additional information no reaction on alpha-, beta- or gamma-cyclodextrin, pullulan and maltose Klebsiella pneumoniae ?
-
 ?
3.2.1.98 additional information substrate and product specificity, overview Klebsiella pneumoniae ?
-
 ?
3.2.1.98 additional information exo action pattern Klebsiella pneumoniae ?
-
 ?
3.2.1.98 additional information no reaction on alpha-, beta- or gamma-cyclodextrin, pullulan and maltose Klebsiella pneumoniae IFO-3321 ?
-
 ?
3.2.1.98 additional information substrate and product specificity, overview Klebsiella pneumoniae IFO-3321 ?
-
 ?
3.2.1.98 additional information exo action pattern Klebsiella pneumoniae IFO-3321 ?
-
 ?
3.2.1.98 starch + H2O soluble starch Klebsiella pneumoniae maltohexaose + ? main product ?
3.2.1.98 starch + H2O waxy-maize starch Klebsiella pneumoniae maltohexaose + ? main product ?

Subunits

EC Number Subunits Comment Organism
3.2.1.98 monomer extracellular enzyme, 1 * 65000, SDS-PAGE Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.98 52
-
 extracellular enzyme Klebsiella pneumoniae
3.2.1.98 60
-
 cell-bound enzyme Klebsiella pneumoniae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.98 additional information
-
 enzyme more thermostable in the presence of Ca2+ Klebsiella pneumoniae
3.2.1.98 50
-
 cell-bound and extracellular enzyme, stable below 50°C, thermostability improved by addition of Ca2+ Klebsiella pneumoniae
3.2.1.98 55
-
 extracellular enzyme, stable up to 55°C in the presence of 2 mM CaCl2 Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.98 6.8
-
 cell-bound enzyme Klebsiella pneumoniae
3.2.1.98 7
-
 extracellular enzyme Klebsiella pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.98 5 10 extracellular enzyme, more than 20% activity at pH 5.0, more than 50% activity at pH 10.0 Klebsiella pneumoniae
3.2.1.98 6 8 extracellular enzyme, more than 80% of maximum activity between pH 6.0 and 8.0 Klebsiella pneumoniae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.98 additional information
-
 pH stability: extracellular enzyme is a little more stable compared with the cell-bound enzyme Klebsiella pneumoniae
3.2.1.98 additional information
-
 extracellular enzyme, relatively wide pH stability Klebsiella pneumoniae
3.2.1.98 3
-
 extracellular enzyme, most of activity lost below pH 3.0 Klebsiella pneumoniae
3.2.1.98 5 10 extracellular enzyme, more than 80% of activity retains Klebsiella pneumoniae
3.2.1.98 6 9 cell-bound enzyme: 20% loss of activity, 60 min, 40°C Klebsiella pneumoniae
3.2.1.98 11
-
 extracellular enzyme, most of activity lost above pH 11.0 Klebsiella pneumoniae