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Literature summary extracted from
- Purification and characterization of human serum biotinidase (1986), J. Biol. Chem., 261, 4268-4275.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.1.12 | medicine | plasma biotinidase activity assay for diagnosis and screen newborn infants for biotinidase deficiency, treatment of inborn metabolism errors with large doses of biotin | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.12 | biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Enterococcus faecalis |  |
| 3.5.1.12 | biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Homo sapiens | |
| 3.5.1.12 | biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Sus scrofa | |
| 3.5.1.12 | CuCl2 | - |
Homo sapiens | |
| 3.5.1.12 | CuCl2 | - |
Rattus rattus | |
| 3.5.1.12 | guanidine hydrochloride | 0.5 M | Homo sapiens | |
| 3.5.1.12 | Urea | 0.5 M | Homo sapiens | |
| 3.5.1.12 | ZnCl2 | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.12 | 0.002 | - |
epsilon-N-biotinyl-L-lysine | human serum | Homo sapiens | |
| 3.5.1.12 | 0.0065 | - |
epsilon-N-biotinyl-L-lysine | human plasma | Homo sapiens | |
| 3.5.1.12 | 0.0077 | - |
epsilon-N-biotinyl-L-lysine | pH 5.5 | Homo sapiens | |
| 3.5.1.12 | 0.0078 | - |
epsilon-N-biotinyl-L-lysine | pH 7 | Homo sapiens | |
| 3.5.1.12 | 0.008 | - |
epsilon-N-biotinyl-L-lysine | serum | Sus scrofa | |
| 3.5.1.12 | 0.01 | - |
epsilon-N-biotinyl-L-lysine | porcine liver | Sus scrofa | |
| 3.5.1.12 | 0.016 | - |
epsilon-N-biotinyl-L-lysine | kidney | Sus scrofa | |
| 3.5.1.12 | 0.0306 | - |
p-(N-biotinylamino)benzoic acid | pH 5.5, D-biotin as inhibitor | Homo sapiens | |
| 3.5.1.12 | 0.0319 | - |
p-(N-biotinylamino)benzoic acid | pH 5.5 | Homo sapiens | |
| 3.5.1.12 | 0.0333 | - |
p-(N-biotinylamino)benzoic acid | pH 7 | Homo sapiens | |
| 3.5.1.12 | 0.0341 | - |
p-(N-biotinylamino)benzoic acid | pH 7, D-biocytin as inhibitor | Homo sapiens | |
| 3.5.1.12 | 0.0685 | - |
N-DL-desthiobiotinyl-p-aminobenzoic acid | pH 7 | Homo sapiens | |
| 3.5.1.12 | 0.0714 | - |
N-DL-desthiobiotinyl-p-aminobenzoic acid | pH 5.5 | Homo sapiens | |
| 3.5.1.12 | 0.0905 | - |
m-(N-biotinylamino)benzoic acid | pH 5.5 | Homo sapiens | |
| 3.5.1.12 | 0.1002 | - |
m-(N-biotinylamino)benzoic acid | - |
Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.1.12 | mitochondrion | - |
Homo sapiens | 5739 | - |
| 3.5.1.12 | mitochondrion | - |
Rattus rattus | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.12 | 68000 | - |
- |
Sus scrofa |
| 3.5.1.12 | 68000 | - |
gel filtration, SDS-PAGE, sedimentation analysis | Homo sapiens |
| 3.5.1.12 | 76000 | - |
- |
Homo sapiens |
| 3.5.1.12 | 76000 | - |
gel filtration, SDS-PAGE | Sus scrofa |
| 3.5.1.12 | 80000 | - |
SDS-PAGE | Homo sapiens |
| 3.5.1.12 | 110000 | - |
gel filtration | Homo sapiens |
| 3.5.1.12 | 115000 | - |
- |
Homo sapiens |
| 3.5.1.12 | 115000 | - |
gel filtration | Sus scrofa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.12 | biotin amide + H2O | Homo sapiens | - |
? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | Sus scrofa | - |
? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | Enterococcus faecalis | - |
? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | Rattus rattus | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Homo sapiens | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Sus scrofa | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Enterococcus faecalis | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Rattus rattus | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.12 | Enterococcus faecalis | - |
- |
- |
| 3.5.1.12 | Homo sapiens | - |
human | - |
| 3.5.1.12 | Rattus rattus | - |
rat | - |
| 3.5.1.12 | Sus scrofa | - |
pig | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.12 | - |
Homo sapiens |
| 3.5.1.12 | - |
Enterococcus faecalis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.12 | blood | serum, plasma, leukocytes | Homo sapiens | - |
| 3.5.1.12 | blood | serum, plasma, leukocytes | Sus scrofa | - |
| 3.5.1.12 | blood | serum, plasma, leukocytes | Rattus rattus | - |
| 3.5.1.12 | liver | - |
Homo sapiens | - |
| 3.5.1.12 | liver | - |
Sus scrofa | - |
| 3.5.1.12 | liver | - |
Rattus rattus | - |
| 3.5.1.12 | small intestine | - |
Homo sapiens | - |
| 3.5.1.12 | small intestine | - |
Rattus rattus | - |
| 3.5.1.12 | stomach | - |
Rattus rattus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.12 | biotin amide + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | - |
Sus scrofa | ? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | - |
Enterococcus faecalis | ? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | - |
Rattus rattus | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Homo sapiens | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Sus scrofa | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Enterococcus faecalis | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Rattus rattus | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Sus scrofa | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Enterococcus faecalis | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Rattus rattus | ? | - |
? | |
| 3.5.1.12 | N-(+)biotinyl-4-aminobenzoic acid + H2O | - |
Homo sapiens | 4-aminobenzoic acid + biotin | - |
? | |
| 3.5.1.12 | N-biotinyl-3-aminobenzoic acid + H2O | - |
Homo sapiens | biotin + 3-aminobenzoic acid | - |
? | |
| 3.5.1.12 | N-biotinyl-3-aminobenzoic acid + H2O | - |
Sus scrofa | biotin + 3-aminobenzoic acid | - |
? | |
| 3.5.1.12 | N-DL-dethiobiotinyl-p-aminobenzoic acid + H2O | - |
Homo sapiens | dethiobiotin + 4-aminobenzoic acid | - |
? | |
| 3.5.1.12 | p-(N-biotinylamino)benzoic acid + H2O | - |
Homo sapiens | biotin + p-aminobenzoic acid | - |
ir | |
| 3.5.1.12 | p-(N-biotinylamino)benzoic acid + H2O | - |
Sus scrofa | biotin + p-aminobenzoic acid | - |
ir | |
| 3.5.1.12 | p-(N-biotinylamino)benzoic acid + H2O | - |
Enterococcus faecalis | biotin + p-aminobenzoic acid | - |
ir | |
| 3.5.1.12 | p-(N-biotinylamino)benzoic acid + H2O | - |
Rattus rattus | biotin + p-aminobenzoic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.12 | monomer | 1 * 68000, SDS-PAGE | Homo sapiens |
| 3.5.1.12 | monomer | 1 * 68000, SDS-PAGE | Sus scrofa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.12 | 60 | 70 | - |
Sus scrofa |
| 3.5.1.12 | 60 | 70 | - |
Rattus rattus |
| 3.5.1.12 | 60 | 70 | 15 min 60°C 60% activity lost, 70°C activity completely lost | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.12 | 4.5 | 6 | with natural substrate biocytin | Homo sapiens |
| 3.5.1.12 | 6 | 7.5 | with synthetic substrates | Homo sapiens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.12 | 5 | 7 | - |
Homo sapiens |
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