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Literature summary extracted from

  • Huynh, M.S.; Snell, E.E.
    Enzymes of vitamin B6 degradation. Purification and properties of two N-acetylamidohydrolases (1985), J. Biol. Chem., 260, 2379-2383.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.5.1.29 stable at room temperature between pH 6 and 10 Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.29 alpha-hydroxyglutarate competitive inhibition Pseudomonas sp.
3.5.1.29 alpha-ketoglutarate
-
 Arthrobacter sp.
3.5.1.29 alpha-ketoglutarate competitive inhibition Pseudomonas sp.
3.5.1.29 citrate competitive inhibition Pseudomonas sp.
3.5.1.29 diphosphate
-
 Arthrobacter sp.
3.5.1.29 diphosphate competitive inhibition Pseudomonas sp.
3.5.1.29 fumarate
-
 Arthrobacter sp.
3.5.1.29 fumarate competitive inhibition Pseudomonas sp.
3.5.1.29 glutamate competitive inhibition Pseudomonas sp.
3.5.1.29 Glutarate competitive inhibition Pseudomonas sp.
3.5.1.29 Hg2+
-
 Pseudomonas sp.
3.5.1.29 Itaconate competitively inhibited by di-and tricarboxylic acids, itaconic acid being most effective, monocarboxylic acids do not inhibit Arthrobacter sp.
3.5.1.29 Itaconate competitively inhibited by di-and tricarboxylic acids, itaconic acid being most effective, monocarboxylic acids do not inhibit Pseudomonas sp.
3.5.1.29 malate competitive inhibition Pseudomonas sp.
3.5.1.29 Maleate competitive inhibition Pseudomonas sp.
3.5.1.29 malonate competitive inhibition Pseudomonas sp.
3.5.1.29 N-acetyl-DL-aspartate
-
 Arthrobacter sp.
3.5.1.29 N-acetyl-DL-aspartate competitive inhibition Pseudomonas sp.
3.5.1.29 oxaloacetate
-
 Arthrobacter sp.
3.5.1.29 oxaloacetate competitive inhibition Pseudomonas sp.
3.5.1.29 phosphate
-
 Arthrobacter sp.
3.5.1.29 phosphate competitive inhibition Pseudomonas sp.
3.5.1.29 SO32- inhibitory effect increases rapidly with time to a constant level, not competitve with substrate Arthrobacter sp.
3.5.1.29 SO32- inhibitory effect increases rapidly with time to a constant level, not competitve with substrate Pseudomonas sp.
3.5.1.29 succinate
-
 Arthrobacter sp.
3.5.1.29 succinate competitive inhibition Pseudomonas sp.
3.5.1.66 Dicarboxylic acids competitive Arthrobacter sp.
3.5.1.66 diphosphate competitive Arthrobacter sp.
3.5.1.66 Hg2+ 0.01 mM, 15 min, 82% activity remains Arthrobacter sp.
3.5.1.66 Itaconic acid competitive, most effective inhibitor, Ki: 0.74 mM Arthrobacter sp.
3.5.1.66 malonate competitive, Ki: 20 mM Arthrobacter sp.
3.5.1.66 additional information no inhibition by: organic protein reagents, 0.1 mM, 15 min, p-chloromercuribenzoate, 1.0 mM Ni2+, 1.0 mM Co2+, 1.0 mM Mn2+, 1.0 mM Mg2+, 1.0 mM Ca2+, 1.0 mM Cu2+, 1.0 mM Cs+, 1.0 mM Li+, SO42-, NO3-, Cl-, 10 mM dithiothreitol, 100 mM monocarboxylic acids Arthrobacter sp.
3.5.1.66 phosphate competitive Arthrobacter sp.
3.5.1.66 sulfite noncompetitive, inhibition by a time-dependent mechanism, addition of succinic semialdehyde protects against sulfite inhibition Arthrobacter sp.
3.5.1.66 Tricarboxylic acids competitive Arthrobacter sp.
3.5.1.66 Zn2+ 1 mM: 40% inhibition Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.29 0.003
-
 2-(Acetamidomethylene)succinate at pH 6.5 and 25°C Pseudomonas sp.
3.5.1.66 additional information
-
 additional information kinetic data Arthrobacter sp.
3.5.1.66 0.025
-
 2-(Hydroxymethyl)-3-(acetamidomethylene)succinate compound B, 25°C, Tris-HCl, pH 7.0 Arthrobacter sp.
3.5.1.66 0.123
-
 2-(Hydroxymethyl)-3-(acetamidomethylene)succinate compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.0 Arthrobacter sp.
3.5.1.66 0.25
-
 2-(Hydroxymethyl)-3-(acetamidomethylene)succinate compound B, Tris-HCl, pH 8.0 Arthrobacter sp.
3.5.1.66 0.56
-
 2-(Hydroxymethyl)-3-(acetamidomethylene)succinate compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.5 Arthrobacter sp.
3.5.1.66 0.68
-
 3-(acetamidomethylene)succinate compound A, 25°C, pH 7.0 Arthrobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.66 additional information some inorganic cations activate Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.29 32500
-
 gel filtration Pseudomonas sp.
3.5.1.66 36600
-
 Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE Arthrobacter sp.
3.5.1.66 205000
-
 Cr-7, gel filtration Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.29 2-(acetamidomethylene)succinate + 2 H2O Pseudomonas sp.
-
 acetate + succinate semialdehyde + NH3 + CO2
-
 ?
3.5.1.29 2-(acetamidomethylene)succinate + 2 H2O Pseudomonas sp. IA
-
 acetate + succinate semialdehyde + NH3 + CO2
-
 ?
3.5.1.66 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O Pseudomonas sp. compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid ?
-
 ?
3.5.1.66 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O Arthrobacter sp. compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid ?
-
 ?
3.5.1.66 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O Arthrobacter sp. involved in pyridoxine degradation ?
-
 ?
3.5.1.66 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O Pseudomonas sp. IA compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid ?
-
 ?
3.5.1.66 additional information Pseudomonas sp. enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen ?
-
 ?
3.5.1.66 additional information Arthrobacter sp. enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen ?
-
 ?
3.5.1.66 additional information Pseudomonas sp. IA enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.29 Arthrobacter sp.
-
 Cr-7
-
3.5.1.29 Pseudomonas sp.
-
 MA-1
-
3.5.1.66 Arthrobacter sp.
-
 Cr-7
-
3.5.1.66 Pseudomonas sp.
-
 IA
-
3.5.1.66 Pseudomonas sp. IA
-
 IA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.29
-
 Pseudomonas sp.
3.5.1.66 Cr-7 Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.29 3.12
-
-
 Pseudomonas sp.
3.5.1.66 additional information
-
-
 Arthrobacter sp.
3.5.1.66 3.79
-
 25°C Arthrobacter sp.

Storage Stability

EC Number Storage Stability Organism
3.5.1.66 Cr-7, -20°C, 0.1 M Tris-HCl, pH 7.0, stable for several months Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.29 2-(acetamidomethylene)succinate + 2 H2O
-
 Pseudomonas sp. acetate + succinate semialdehyde + NH3 + CO2
-
 ?
3.5.1.29 2-(acetamidomethylene)succinate + 2 H2O
-
 Pseudomonas sp. acetate + succinate semialdehyde + NH3 + CO2 same products as formed by acid hydrolysis ir
3.5.1.29 2-(acetamidomethylene)succinate + 2 H2O
-
 Pseudomonas sp. IA acetate + succinate semialdehyde + NH3 + CO2
-
 ?
3.5.1.29 2-(acetamidomethylene)succinate + 2 H2O
-
 Pseudomonas sp. IA acetate + succinate semialdehyde + NH3 + CO2 same products as formed by acid hydrolysis ir
3.5.1.66 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid Pseudomonas sp. acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
 ?
3.5.1.66 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid Arthrobacter sp. acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
 ?
3.5.1.66 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid Pseudomonas sp. IA acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
 ?
3.5.1.66 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid Pseudomonas sp. ?
-
 ?
3.5.1.66 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid Arthrobacter sp. ?
-
 ?
3.5.1.66 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O involved in pyridoxine degradation Arthrobacter sp. ?
-
 ?
3.5.1.66 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid Pseudomonas sp. IA ?
-
 ?
3.5.1.66 3-(acetamidomethylene)succinate + H2O compound A, alpha-(N-acetylaminomethylene)succinic acid: poor substrate Arthrobacter sp. acetate + 4-oxobutanoate + NH3 + CO2
-
 ?
3.5.1.66 additional information high substrate specificity Arthrobacter sp. ?
-
 ?
3.5.1.66 additional information enzyme highly specific, inducible amidohydrolase Arthrobacter sp. ?
-
 ?
3.5.1.66 additional information enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen Pseudomonas sp. ?
-
 ?
3.5.1.66 additional information enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen Arthrobacter sp. ?
-
 ?
3.5.1.66 additional information enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen Pseudomonas sp. IA ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.29 monomer
-
 Arthrobacter sp.
3.5.1.29 monomer SDS-PAGE Pseudomonas sp.
3.5.1.66 multimer Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE Arthrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.1.29 compound A hydrolase
-
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.29 25
-
-
 Pseudomonas sp.
3.5.1.66 25
-
 assay at Arthrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.29 70 90 at pH 8 and 70°C stable for 1 min, loses activity completely in 1 min at 90°C Pseudomonas sp.
3.5.1.66 70
-
 pH 8.0, 1 min, stable at Arthrobacter sp.
3.5.1.66 100
-
 1 min, 65% activity retained Arthrobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.29 1.67
-
 2-(Acetamidomethylene)succinate
-
 Pseudomonas sp.
3.5.1.66 2.3
-
 2-(Hydroxymethyl)-3-(acetamidomethylene)succinate turnover no./subunit, 25°C Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.29 6.5 7.5 pH 6.5 in 50 mM sodium phosphate, MES and HEPES buffers, pH 7.5 in Tris buffer Pseudomonas sp.
3.5.1.66 additional information
-
 pI: 4.2, effect of buffer on pH optimum Arthrobacter sp.
3.5.1.66 6 7
-
 Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.29 6 10 most efficient between pH 6.0 and 7.0 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.29 6 10 loses activity rapidly between 4.0 and 5.0 Pseudomonas sp.
3.5.1.66 4 5 rapid loss of activity Arthrobacter sp.
3.5.1.66 6 10 stable at room temperature Arthrobacter sp.
3.5.1.66 8
-
 70°C, 1 min, stable at Arthrobacter sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.66 0.74
-
 Itaconic acid competitive, most effective inhibitor Arthrobacter sp.
3.5.1.66 20
-
 malonate competitive Arthrobacter sp.