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Literature summary extracted from

  • Suzuki, O.; Ishikawa, Y.; Miyazaki, K.; Izu, K.; Matsumoto, T.
    Acetylputrescine deacetylase from Micrococcus luteus K-11 (1986), Biochim. Biophys. Acta, 882, 140-142.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.62 8-hydroxyquinoline very slight inhibition, 1 mM: 2% inhibition Micrococcus luteus
3.5.1.62 Ca2+ 1 mM: 6.2% inhibition Micrococcus luteus
3.5.1.62 CaCl2 1 mM: 6.2% inhibition Micrococcus luteus
3.5.1.62 Cu2+ 1 mM: 74.4% inhibition Micrococcus luteus
3.5.1.62 CuCl2 1 mM: 74.4% inhibition Micrococcus luteus
3.5.1.62 iodoacetamide very slight inhibition, 1 mM: 2% inhibition Micrococcus luteus
3.5.1.62 additional information no or very slight inhibition by -SH blocking agents Micrococcus luteus
3.5.1.62 Ni2+ 1 mM: 66.8% inhibition Micrococcus luteus
3.5.1.62 NiCl2 1 mM: 66.8% inhibition Micrococcus luteus
3.5.1.62 p-chloromercuribenzoic acid very slight inhibition, 1 mM: 2% inhibition Micrococcus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.62 0.321
-
 N-acetylputrescine
-
 Micrococcus luteus
3.5.1.62 0.377
-
 N8-Acetylspermidine
-
 Micrococcus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.62 Fe3+ 1 mM: slight activation Micrococcus luteus
3.5.1.62 FeCl3 1 mM: slight activation Micrococcus luteus
3.5.1.62 Mn2+ 1 mM: slight activation Micrococcus luteus
3.5.1.62 MnCl2 1 mM: slight activation Micrococcus luteus
3.5.1.62 additional information Mg2+ 1 mM: no influence Micrococcus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.62 115000
-
 gel filtration Micrococcus luteus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.62 Micrococcus luteus
-
-
-
3.5.1.62 Micrococcus luteus K-11
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.62 strain K-11, partial Micrococcus luteus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.62 0.48
-
 substrate: acetylputrescine Micrococcus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.62 acetylcadaverine + H2O 12% of activity compared to acetylputrescine Micrococcus luteus acetate + cadaverine
-
 ?
3.5.1.62 acetylcadaverine + H2O 12% of activity compared to acetylputrescine Micrococcus luteus K-11 acetate + cadaverine
-
 ?
3.5.1.62 additional information N1-acetylspermidine, N1-acetylspermine: no substrates Micrococcus luteus ?
-
 ?
3.5.1.62 additional information N1-acetylspermidine, N1-acetylspermine: no substrates Micrococcus luteus K-11 ?
-
 ?
3.5.1.62 N-acetylputrescine + H2O enzyme induction by N-acetylputrescine Micrococcus luteus acetate + putrescine
-
 ?
3.5.1.62 N-acetylputrescine + H2O enzyme induction by N-acetylputrescine Micrococcus luteus K-11 acetate + putrescine
-
 ?
3.5.1.62 N8-acetylspermidine + H2O 85.2% of activity compared to acetylputrescine Micrococcus luteus acetate + spermidine
-
 ?
3.5.1.62 N8-acetylspermidine + H2O 85.2% of activity compared to acetylputrescine Micrococcus luteus K-11 acetate + spermidine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.62 37
-
 assay at Micrococcus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.62 8
-
 substrate: acetylputrescine Micrococcus luteus
3.5.1.62 9
-
 substrate: N8-acetylspermidine Micrococcus luteus