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Literature summary extracted from
- Allantoate amidinohydrolase (allantoicase) from Chlamydomonas reinhardtii: Its Purification and catalytic and molecular characterization (2000), Arch. Biochem. Biophys., 378, 340-348.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.3.4 | Allantoate | with (-)-ureidoglycolate as substrate | Chlamydomonas reinhardtii |  |
| 3.5.3.4 | Cu2+ | - |
Chlamydomonas reinhardtii | |
| 3.5.3.4 | diethyldicarbonate | - |
Chlamydomonas reinhardtii | |
| 3.5.3.4 | EDTA | - |
Chlamydomonas reinhardtii | |
| 3.5.3.4 | glycolic acid | - |
Chlamydomonas reinhardtii | |
| 3.5.3.4 | iodoacetate | slight | Chlamydomonas reinhardtii | |
| 3.5.3.4 | N-Acetylimidazole | - |
Chlamydomonas reinhardtii | |
| 3.5.3.4 | NH4+ | - |
Chlamydomonas reinhardtii | |
| 3.5.3.4 | p-hydroxymercuribenzoate | - |
Chlamydomonas reinhardtii | |
| 3.5.3.4 | Urea | - |
Chlamydomonas reinhardtii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.3.4 | 0.7 | - |
(-)-ureidoglycolate | - |
Chlamydomonas reinhardtii | |
| 3.5.3.4 | 2 | - |
Allantoate | - |
Chlamydomonas reinhardtii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.3.4 | Mn2+ | restores activity after treatment with EDTA | Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.4 | 34000 | - |
6 * 34000, SDS-PAGE after 2-mercaptoethanol treatment | Chlamydomonas reinhardtii |
| 3.5.3.4 | 200000 | - |
gel filtration | Chlamydomonas reinhardtii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.3.4 | Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.3.4 | - |
Chlamydomonas reinhardtii |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.5.3.4 | allantoate + H2O = (S)-ureidoglycolate + urea | thermodynamic data | Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.4 | 185 | - |
- |
Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.3.4 | (-)-ureidoglycolate | - |
Chlamydomonas reinhardtii | glyoxylate + urea | - |
? | |
| 3.5.3.4 | allantoate + H2O | - |
Chlamydomonas reinhardtii | (-)-ureidoglycolate + urea | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.3.4 | hexamer | 6 * 34000, SDS-PAGE after 2-mercaptoethanol treatment | Chlamydomonas reinhardtii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.4 | 60 | - |
- |
Chlamydomonas reinhardtii |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.3.4 | 750 | - |
Allantoate | - |
Chlamydomonas reinhardtii | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.4 | 6.5 | - |
with allantoate | Chlamydomonas reinhardtii |
| 3.5.3.4 | 8 | - |
with (-)-ureidoglycolate | Chlamydomonas reinhardtii |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.4 | 6.5 | 10 | - |
Chlamydomonas reinhardtii |
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