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Literature summary extracted from

  • Rabin, B.A.; Chase, J.W.
    DNA ligase from Drosophila melanogaster embryos. Substrate specificity and mechanism of action (1987), J. Biol. Chem., 262, 14105-14111.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 dATP
-
Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.001
-
ATP p(dT)20 annealed with poly(dA) Drosophila melanogaster
6.5.1.1 0.0016
-
ATP
-
Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1 DNA ligase II Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.5.1.1 embryo
-
Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 5'-O-(3-Thio)adenosine triphosphate + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Drosophila melanogaster 5'-O-(3-Thio)adenosine monophosphate + diphosphate + (deoxyribonucleotide)n+m
-
?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m overview: activity of DNA ligase on ribo- and deoxyribopolymer substrates Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m reverse reaction: incubation of superhelical closed circular DNA molecules with the enzyme and AMP results in the production of a population of DNA molecules which have lost most of their superhelical density Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m p(dT)7U can be joined when annealed with poly(dA), joining of oligonucleotides containing a single mismatched nucleotide at their 3'-hydroxyl termini, as well as DNA containing short, complementary 5'-protruding ends, and in the presence of glycol 6000, blunt-ended duplex DNA Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m in addition to joining DNA to DNA the enzyme can join the 5'-phosphoryl terminus of RNA to the 3'-hydroxyl terminus of DNA or RNA, when they are annealed with DNA Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
?

Subunits

EC Number Subunits Comment Organism
6.5.1.1 monomer
-
Drosophila melanogaster