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Literature summary extracted from

  • Pan, F.; Lee, H.H.; Pai, S.H.; Yu, T.C.; Guoo, J.Y.; Duh, G.M.
    Multiple molecular forms of cysteinyl-tRNA synthetase from rat liver: Purification and subunit structure (1976), Biochim. Biophys. Acta, 452, 271-283.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.16 120000
-
 2 * 120000, SDS-PAGE in presence of 2-mercaptoethanol, enzyme form CRS-2 Rattus norvegicus
6.1.1.16 122000
-
 gel filtration, enzyme form, CRS-1 Rattus norvegicus
6.1.1.16 127000
-
 sucrose density gradient centrifugation, enzyme form CRS-2 Rattus norvegicus
6.1.1.16 230000 240000 gel filtration, PAGE on gels of various porosities, enzyme form CRS-2 Rattus norvegicus
6.1.1.16 270000 300000 gel filtration, enzyme form CRS-3 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.16 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.16 multiple molecular forms: CRS-1, CRS-2, CRS-3 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.16 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.16 1.2
-
 enzyme form CRS-1 Rattus norvegicus
6.1.1.16 3.29
-
 enzyme form CRS-2 Rattus norvegicus
6.1.1.16 6.25
-
 enzyme form CRS-3 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
6.1.1.16 4°C, 50% glycerol, 0.5 mM DTT, 3 mM L-cysteine Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.16 ATP + L-cysteine + tRNACys
-
 Rattus norvegicus AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.16 dimer 2 * 120000, SDS-PAGE in presence of 2-mercaptoethanol, enzyme form CRS-2 Rattus norvegicus