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Literature summary extracted from

  • Takahashi, M.; Senshu, M.
    Two distinct DNA ligases from Drosophila melanogaster embryos (1987), FEBS Lett., 213, 345-352.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.5.1.1 dithiothreitol required for optimal activity of DNA ligase I, but not for DNA ligase II activity Drosophila melanogaster
6.5.1.1 polyvinyl alcohol required for optimal activity of DNA ligase I but not for DNA ligase II activity Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 NEM
-
 Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.0027
-
 ATP DNA ligase I Drosophila melanogaster
6.5.1.1 0.03
-
 ATP DNA ligase II Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.5.1.1 cytoplasm DNA ligase I Drosophila melanogaster 5737
-
6.5.1.1 nucleus DNA ligase II Drosophila melanogaster 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 additional information DNA ligase I is active at low salt concentrations, 0-30 mM KCl, DNA ligase II is active at high salt concentrations, 50-100 mM KCl Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Drosophila melanogaster
-
 DNA ligase I and II
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.5.1.1 embryo
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase I and II ligate 5'-phosphoryl and 3'-hydroxyl groups in oligo(dT) in the presence of poly(dA). DNA ligase II can join oligo(dT)*poly(rA) Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
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