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Literature summary extracted from
- Partial purification and characterization of an aspartate racemase from Streptococcus faecalis (1972), J. Biol. Chem., 247, 5103-5106.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.13 | additional information | removal of some divalent cation by EDTA gives slight increase in activity | Enterococcus faecalis | |
| 5.1.1.13 | pyridoxal phosphate | racemase contains bound pyridoxal phosphate | Enterococcus faecalis |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.13 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Enterococcus faecalis | |
| 5.1.1.13 | Mn2+ | - |
Enterococcus faecalis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.13 | 25 | - |
L-Asp | - |
Enterococcus faecalis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.1.13 | Enterococcus faecalis | - |
strain ATCC 9790, strain IAM 10065, no activity in strain IAM 10067 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.1.13 | - |
Enterococcus faecalis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.13 | L-Ala | at half the rate of Asp | Enterococcus faecalis | D-Ala | - |
? | |
| 5.1.1.13 | L-Asp | - |
Enterococcus faecalis | D-Asp | - |
? | |
| 5.1.1.13 | additional information | no racemase activity with Glu | Enterococcus faecalis | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.13 | 100 | - |
thermal stability shows a bimodal dependence on pH, with minimum stability at pH 7.5, and remarkably stability at pH 6 and pH 8-9. 10 min, pH 8, 66% loss of activity | Enterococcus faecalis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.13 | 6 | 9 | - |
Enterococcus faecalis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.13 | 6 | 9 | thermal stability shows a bimodal dependence on pH, with minimum stability at pH 7.5, and remarkably stability at pH 6 and pH 8-9. 10 min, pH 8, 66% loss of activity | Enterococcus faecalis |
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