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Literature summary extracted from
- Microbial metabolism of quinoline and related compounds. XIV. Purification and properties of 1H-3-hydroxy-4-oxoquinoline oxygenase, a new extradiol cleavage enzyme from Pseudomonas putida strain 33/1 (1992), Biol. Chem. Hoppe-Seyler, 373, 343-349.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.47 | 1,10-phenanthroline | - |
Pseudomonas putida |  |
| 1.13.11.47 | 2,2'-dipyridyl | - |
Pseudomonas putida | |
| 1.13.11.47 | Cu2+ | - |
Pseudomonas putida | |
| 1.13.11.47 | Hg2+ | - |
Pseudomonas putida | |
| 1.13.11.47 | iodoacetate | - |
Pseudomonas putida | |
| 1.13.11.47 | KCN | - |
Pseudomonas putida | |
| 1.13.11.47 | KSCN | - |
Pseudomonas putida | |
| 1.13.11.47 | PCMB | - |
Pseudomonas putida | |
| 1.13.11.47 | Zn2+ | - |
Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.47 | 0.024 | - |
1H-3-Hydroxy-4-oxoquinoline | - |
Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.47 | 26000 | - |
gel filtration | Pseudomonas putida |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.47 | Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.47 | - |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.47 | 9.9 | - |
- |
Pseudomonas putida |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.13.11.47 | -20°C, stable for 3 days | Pseudomonas putida |
| 1.13.11.47 | 4°C, 15% loss of activity after 2 days | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.47 | 1H-3-Hydroxy-4-oxoquinoline + O2 | - |
Pseudomonas putida | N-Formylanthranilate + CO | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.47 | monomer | 1 * 26000, SDS-PAGE | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.47 | 8 | - |
- |
Pseudomonas putida |
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