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Literature summary extracted from

  • Schläfli Oppenberg, H.R.; Chen, G.; Leisinger, T.; Cook, A.M.
    Regulation of the degradative pathways from 4-toluenesulphonate and 4-toluenecarboxylate to protocatechuate in Comamonas testosteroni T-2 (1995), Microbiology, 141, 1891-1899.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.1.62 unstable in cell extracts Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O Comamonas testosteroni
-
 4-sulfobenzoate + NADH
-
 ?
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O Comamonas testosteroni T-2
-
 4-sulfobenzoate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.257 Comamonas testosteroni
-
-
-
1.1.1.257 Comamonas testosteroni T-2
-
-
-
1.2.1.62 Comamonas testosteroni
-
-
-
1.2.1.62 Comamonas testosteroni T-2
-
-
-
1.14.12.8 Comamonas testosteroni
-
 T-2
-
1.14.12.8 Comamonas testosteroni T-2
-
 T-2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.257 cell culture
-
 Comamonas testosteroni
-
1.2.1.62 cell culture
-
 Comamonas testosteroni
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.8 0.012
-
 for enzyme system grown on sulfobenzoate Comamonas testosteroni
1.14.12.8 0.024
-
 for enzyme system grown on toluenesulfonate Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.257 4-(hydroxymethyl)benzenesulfonate + NAD+ part of a specific transport system that mediates uptake of toluenesulfonat/toluenecarboxylate Comamonas testosteroni 4-formylbenezenesulfonate + NADH
-
 r
1.1.1.257 4-(hydroxymethyl)benzenesulfonate + NAD+ part of a specific transport system that mediates uptake of toluenesulfonat/toluenecarboxylate Comamonas testosteroni T-2 4-formylbenezenesulfonate + NADH
-
 r
1.1.1.257 4-carboxybenzaldehyde + NADH part of a specific transport system that mediates uptake of toluenesulfonat/toluenecarboxylate pathway of degradation Comamonas testosteroni 4-carboxybenzyl alcohol + NAD+
-
 r
1.1.1.257 4-carboxybenzaldehyde + NADH part of a specific transport system that mediates uptake of toluenesulfonat/toluenecarboxylate pathway of degradation Comamonas testosteroni T-2 4-carboxybenzyl alcohol + NAD+
-
 r
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O
-
 Comamonas testosteroni 4-sulfobenzoate + NADH
-
 ?
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O
-
 Comamonas testosteroni T-2 4-sulfobenzoate + NADH
-
 ?
1.14.12.8 4-sulfobenzoate + NADH + O2 the oxygenase component was expressed at high levels, no expression of a specific reductase activity linked to synthesis of the oxygenase could be detected Comamonas testosteroni 3,4-dihydroxybenzoate + sulfite + NAD+
-
 ?
1.14.12.8 4-sulfobenzoate + NADH + O2 the oxygenase component was expressed at high levels, no expression of a specific reductase activity linked to synthesis of the oxygenase could be detected Comamonas testosteroni T-2 3,4-dihydroxybenzoate + sulfite + NAD+
-
 ?
1.14.12.8 4-toluenesulfonate + NADH + O2 the oxygenase component was expressed at high levels, no expression of a specific reductase activity linked to synthesis of the oxygenase could be detected Comamonas testosteroni ?
-
 ?
1.14.12.8 4-toluenesulfonate + NADH + O2 the oxygenase component was expressed at high levels, no expression of a specific reductase activity linked to synthesis of the oxygenase could be detected Comamonas testosteroni T-2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.257 sulfobenzyl alcohol dehydrogenase
-
 Comamonas testosteroni
1.2.1.62 SYDDH
-
 Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.257 NAD+
-
 Comamonas testosteroni
1.2.1.62 NAD+
-
 Comamonas testosteroni