EC Number | General Stability | Organism |
---|---|---|
1.2.1.33 | aldopantoate stabilizes against heat | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.33 | (R)-4-dehydropantoate | - |
Pseudomonas sp. | |
1.2.1.33 | NAD+ | above 0.3 mM | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.84 | 0.15 | 0.2 | 3,3-dimethyl-D-malate | - |
Pseudomonas sp. | |
1.1.1.84 | 0.27 | - |
NAD+ | - |
Pseudomonas sp. | |
1.1.1.84 | 0.33 | - |
3-(n-Propyl)malate | - |
Pseudomonas sp. | |
1.1.1.84 | 8.9 | 11 | D-malate | - |
Pseudomonas sp. | |
1.2.1.33 | 0.07 | - |
NAD+ | - |
Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.84 | Co2+ | Mn2+ or Co2+ required | Pseudomonas sp. | |
1.1.1.84 | K+ | K+ or NH4+ required | Pseudomonas sp. | |
1.1.1.84 | Mn2+ | Mn2+ or Co2+ required | Pseudomonas sp. | |
1.1.1.84 | NH4+ | K+ or NH4+ required | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.84 | (R)-3,3-dimethylmalate + NAD+ | Pseudomonas sp. | - |
3-methyl-2-oxobutanoate + CO2 + NADH | - |
r | |
1.1.1.84 | (R)-3,3-dimethylmalate + NAD+ | Pseudomonas sp. P-2 | - |
3-methyl-2-oxobutanoate + CO2 + NADH | - |
r | |
1.2.1.33 | (R)-4-dehydropantoate + NAD+ | Pseudomonas sp. | enzyme required for degradation of pantothenate to alpha-ketoisovalerate | (R)-3,3-dimethylmalate + NADH | - |
? | |
1.2.1.33 | (R)-4-dehydropantoate + NAD+ | Pseudomonas sp. P-2 | enzyme required for degradation of pantothenate to alpha-ketoisovalerate | (R)-3,3-dimethylmalate + NADH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.84 | Pseudomonas sp. | - |
P-2 | - |
1.1.1.84 | Pseudomonas sp. P-2 | - |
P-2 | - |
1.2.1.33 | Pseudomonas sp. | - |
P-2 | - |
1.2.1.33 | Pseudomonas sp. P-2 | - |
P-2 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.84 | - |
Pseudomonas sp. |
1.2.1.33 | P-2, partial | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.84 | 4.1 | - |
- |
Pseudomonas sp. |
1.2.1.33 | 0.4 | - |
- |
Pseudomonas sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.84 | 50 days, 0°C | Pseudomonas sp. |
1.2.1.33 | 0°C, 0.1 M potassium phosphate pH 7.5, 0.015 M mercaptoethanol, 3 days | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.84 | (R)-3,3-dimethylmalate + NAD+ | - |
Pseudomonas sp. | 3-methyl-2-oxobutanoate + CO2 + NADH | - |
r | |
1.1.1.84 | (R)-3,3-dimethylmalate + NAD+ | - |
Pseudomonas sp. P-2 | 3-methyl-2-oxobutanoate + CO2 + NADH | - |
r | |
1.1.1.84 | 3-(n-propyl)malate + NAD+ | - |
Pseudomonas sp. | 2-oxopentanoate + CO2 + NADH | - |
? | |
1.1.1.84 | 3-(n-propyl)malate + NAD+ | - |
Pseudomonas sp. P-2 | 2-oxopentanoate + CO2 + NADH | - |
? | |
1.1.1.84 | D-malate + NAD+ | - |
Pseudomonas sp. | pyruvate + CO2 + NADH | - |
? | |
1.1.1.84 | D-malate + NAD+ | - |
Pseudomonas sp. P-2 | pyruvate + CO2 + NADH | - |
? | |
1.2.1.33 | (R)-4-dehydropantoate + NAD+ | enzyme required for degradation of pantothenate to alpha-ketoisovalerate | Pseudomonas sp. | (R)-3,3-dimethylmalate + NADH | - |
? | |
1.2.1.33 | (R)-4-dehydropantoate + NAD+ | enzyme required for degradation of pantothenate to alpha-ketoisovalerate | Pseudomonas sp. P-2 | (R)-3,3-dimethylmalate + NADH | - |
? | |
1.2.1.33 | (R)-4-dehydropantoate + NAD+ + H2O | - |
Pseudomonas sp. | (R)-3,3-dimethylmalate + NADH | - |
? | |
1.2.1.33 | (R)-4-dehydropantoate + NAD+ + H2O | - |
Pseudomonas sp. P-2 | (R)-3,3-dimethylmalate + NADH | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.84 | 60 | - |
5 min, 40% loss of activity | Pseudomonas sp. |
1.2.1.33 | 50 | - |
4 min in presence of aldopantoate: no loss of activity, 1 min in absence of aldopantoate, complete loss of activity | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.84 | 8 | 8.5 | substrates 3,3-dimethylmalate + NAD+ | Pseudomonas sp. |
1.2.1.33 | 9 | - |
or higher, activity increases from pH 7.0 to 9.5 | Pseudomonas sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.33 | 7 | 9.5 | activity increases from pH 7.0 to 9.5 | Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.84 | NAD+ | - |
Pseudomonas sp. | |
1.2.1.33 | additional information | no diffusible cofactors found | Pseudomonas sp. |