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Literature summary extracted from

  • Morisseau, C.; Archelas, A.; Guitton, C.; Faucher, D.; Furstoss, R.; Baratti, J.C.
    Purification and characterization of a highly enantioselective epoxide hydrolase from Aspergillus niger (1999), Eur. J. Biochem., 263, 386-395.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.3.2.10 synthesis the enzyme may be a good biocatalyst for the preparation of enantiopure epoxides or diols Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.3.2.10 4-hydroxymercuribenzoate
-
Aspergillus niger
3.3.2.10 cis-limonene epoxide weak Aspergillus niger
3.3.2.10 dithionitrobenzene
-
Aspergillus niger
3.3.2.10 epichlorhydrin weak Aspergillus niger
3.3.2.10 geraniol derivatives weak Aspergillus niger
3.3.2.10 H2O2
-
Aspergillus niger
3.3.2.10 HgCl2
-
Aspergillus niger
3.3.2.10 iodoacetamide
-
Aspergillus niger
3.3.2.10 m-chloroperbenzoic acid
-
Aspergillus niger
3.3.2.10 omega-bromo-nitro-acetophenone
-
Aspergillus niger
3.3.2.10 trans-limonene epoxide weak Aspergillus niger
3.3.2.10 trichloropropene oxide
-
Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.10 0.6
-
(R)-p-nitrostyrene oxide
-
Aspergillus niger
3.3.2.10 1
-
(R,S)-4-nitrostyrene oxide
-
Aspergillus niger
3.3.2.10 3.7
-
(S)-4-nitrostyrene oxide
-
Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.3.2.10 45000
-
4 * 45000, SDS-PAGE Aspergillus niger
3.3.2.10 185000
-
gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.10 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.10
-
Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3.2.10 0.102
-
-
Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.10 (R)-p-nitrostyrene oxide + H2O
-
Aspergillus niger 1-(4-nitrophenyl)ethane-1,2-diol
-
?
3.3.2.10 (R,S)-4-nitrostyrene oxide + H2O
-
Aspergillus niger 1-(4-nitrophenyl)ethane-1,2-diol
-
?
3.3.2.10 (S)-4-nitrostyrene oxide
-
Aspergillus niger 1-(4-nitrophenyl)ethane-1,2-diol
-
?
3.3.2.10 p-nitrostyrene oxide + H2O
-
Aspergillus niger ?
-
?

Subunits

EC Number Subunits Comment Organism
3.3.2.10 tetramer 4 * 45000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.3.2.10 40
-
-
Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.3.2.10 15 45 15°C: about 50% of maximal activity, 45°C: about 65% of maximal activity Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3.2.10 1.7
-
(S)-4-nitrostyrene oxide
-
Aspergillus niger
3.3.2.10 13.9
-
(R,S)-4-nitrostyrene oxide
-
Aspergillus niger
3.3.2.10 16.4
-
(R)-p-nitrostyrene oxide
-
Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.10 7
-
-
Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.3.2.10 5.5 8 pH 5.5: about 35% of maximal activity, pH 8.0: about 45% of maximal activity Aspergillus niger