Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Raha, S.K.; Roy, S.K.; Dey, S.K.; Chakrabarty, S.L.
    Purification and properties of an L-asparaginase from Cylindrocarpon obtusisporum MB-10 (1990), Biochem. Int., 21, 987-1000.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.1 bovine serum albumin slightly enhances activity Neonectria obtusispora
3.5.1.1 EDTA enhances activity Neonectria obtusispora
3.5.1.1 GSH enhances activity Neonectria obtusispora
3.5.1.1 L-Cys enhances activity Neonectria obtusispora
3.5.1.1 L-His enhances activity Neonectria obtusispora

General Stability

EC Number General Stability Organism
3.5.1.1 stable to repeated freezing and thawing Neonectria obtusispora

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 Cu2+
-
 Neonectria obtusispora
3.5.1.1 D-Asn
-
 Neonectria obtusispora
3.5.1.1 Fe2+
-
 Neonectria obtusispora
3.5.1.1 Hg2+
-
 Neonectria obtusispora
3.5.1.1 iodoacetamide
-
 Neonectria obtusispora
3.5.1.1 Ni2+
-
 Neonectria obtusispora
3.5.1.1 PCMB
-
 Neonectria obtusispora
3.5.1.1 SDS
-
 Neonectria obtusispora
3.5.1.1 Triton X-100
-
 Neonectria obtusispora
3.5.1.1 Urea
-
 Neonectria obtusispora
3.5.1.1 Zn2+ slight inhibition Neonectria obtusispora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 1
-
 L-asparagine
-
 Neonectria obtusispora

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 CN- enhances activity Neonectria obtusispora
3.5.1.1 I- enhances activity Neonectria obtusispora
3.5.1.1 additional information enzyme is not a metalloprotein Neonectria obtusispora
3.5.1.1 PO43- enhances activity Neonectria obtusispora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 216000
-
 gel filtration Neonectria obtusispora
3.5.1.1 543000
-
 4 * 543000, SDS-PAGE Neonectria obtusispora

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-Asn + H2O Neonectria obtusispora constitutive enzyme L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O Neonectria obtusispora MB-20 constitutive enzyme L-Asp + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Neonectria obtusispora
-
 MB-20
-
3.5.1.1 Neonectria obtusispora MB-20
-
 MB-20
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.1 glycoprotein contains 37.3% w/w carbohydrate Neonectria obtusispora

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
 Neonectria obtusispora

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 additional information
-
-
 Neonectria obtusispora

Storage Stability

EC Number Storage Stability Organism
3.5.1.1 -20°C, stable Neonectria obtusispora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 D-Asn + H2O no activity Neonectria obtusispora D-Asp + NH3
-
 ?
3.5.1.1 D-Asn + H2O no activity Neonectria obtusispora MB-20 D-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Neonectria obtusispora L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O constitutive enzyme Neonectria obtusispora L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Neonectria obtusispora MB-20 L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O constitutive enzyme Neonectria obtusispora MB-20 L-Asp + NH3
-
 ?
3.5.1.1 L-Gln + H2O no activity Neonectria obtusispora L-Glu + NH3
-
 ?
3.5.1.1 L-Gln + H2O no activity Neonectria obtusispora MB-20 L-Glu + NH3
-
 ?
3.5.1.1 poly-L-asparagine + H2O at 21.7% of the activity with L-Asn Neonectria obtusispora ?
-
 ?
3.5.1.1 poly-L-asparagine + H2O at 21.7% of the activity with L-Asn Neonectria obtusispora MB-20 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 tetramer 4 * 543000, SDS-PAGE Neonectria obtusispora

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 37
-
-
 Neonectria obtusispora

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.1 20 42 20°C: about 30% of maximal activity, 42°C: about 35% of maximal activity Neonectria obtusispora

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.1 37
-
 30 min, stable Neonectria obtusispora
3.5.1.1 40
-
 30 min, 20% loss of activity Neonectria obtusispora
3.5.1.1 50
-
 30 min, complete loss of activity Neonectria obtusispora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 7.4
-
-
 Neonectria obtusispora