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Literature summary extracted from

  • Engelhardt, G.; Wallnoefer, P.R.
    Purification and properties of an aryl acylamidase of Bacillus sphaericus, catalyzing the hydrolysis of various phenylamide herbicides and fungicides (1973), Appl. Microbiol., 26, 709-718.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.5.1.13 urea, 6 M, irreversible inactivation Lysinibacillus sphaericus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.13 3,4-dichloroaniline
-
 Lysinibacillus sphaericus
3.5.1.13 Ag+ 0.1 mM, 6% inhibition Lysinibacillus sphaericus
3.5.1.13 Cu2+ 0.1 mM, 9.5% inhibition Lysinibacillus sphaericus
3.5.1.13 HgCl2 0.1 mM, 98% inhibition Lysinibacillus sphaericus
3.5.1.13 iodoacetamide
-
 Lysinibacillus sphaericus
3.5.1.13 PCMB
-
 Lysinibacillus sphaericus
3.5.1.13 puromycin competitive inhibition of hydrolysis L-Ala-4-nitroanilide Lysinibacillus sphaericus
3.5.1.13 Sodium arsenate
-
 Lysinibacillus sphaericus
3.5.1.13 Urea 6 M, irreversible inactivation Lysinibacillus sphaericus
3.5.1.13 Zn2+ 0.1 mM, 51% inhibition Lysinibacillus sphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.13 0.002
-
 3-(3,4-dichlorophenyl)-1-methoxy-1-methylurea
-
 Lysinibacillus sphaericus
3.5.1.13 0.03
-
 2,5-dimethylfuran-3-carboxanilide
-
 Lysinibacillus sphaericus
3.5.1.13 0.09
-
 L-Ala 4-nitroanilide
-
 Lysinibacillus sphaericus
3.5.1.13 0.2
-
 L-Leu 4-nitroanilide
-
 Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.13 75000
-
 gel filtration Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.13 additional information Lysinibacillus sphaericus inducible by various phenylamides of the acylanilide, phenylcarbamate, and methoxysubstituted phenylurea type ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.13 Lysinibacillus sphaericus
-
 ATCC 12123
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.13
-
 Lysinibacillus sphaericus

Storage Stability

EC Number Storage Stability Organism
3.5.1.13 -15°C, stable for 3 months Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.13 2,5-dimethylfuran-3-carboxanilide + H2O
-
 Lysinibacillus sphaericus ?
-
 ?
3.5.1.13 3-(3,4-dichlorophenyl)-1-methoxy-1-methylurea + H2O i.e. linuron Lysinibacillus sphaericus 3,4-dichloroaniline + ?
-
 ?
3.5.1.13 L-Ala-4-nitroanilide + H2O
-
 Lysinibacillus sphaericus Ala + p-nitroaniline
-
 ?
3.5.1.13 L-Leu p-nitroanilide + H2O
-
 Lysinibacillus sphaericus Leu + p-nitroaniline
-
 ?
3.5.1.13 additional information inducible by various phenylamides of the acylanilide, phenylcarbamate, and methoxysubstituted phenylurea type Lysinibacillus sphaericus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.13 40
-
 9.5 min, 50% loss of activity Lysinibacillus sphaericus
3.5.1.13 45
-
 2 min, 50% loss of activity Lysinibacillus sphaericus
3.5.1.13 50
-
 0.5 min, 50% loss of activity Lysinibacillus sphaericus