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Literature summary extracted from
- Regulation of glutaminase B in Escherichia coli. I. Purification, properties, and cold lability (1976), J. Biol. Chem., 251, 3447-3456.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.5.1.2 | significant loss of activity upon freezing at -20°C and -80°C in presence and absence of 20% glycerol | Escherichia coli |
| 3.5.1.2 | sodium borate is the most effective stabilizing agent against cold inactivation | Escherichia coli |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.2 | 90000 | - |
glutaminase B, gel filtration | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.2 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.2 | glutaminase B | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.2 | additional information | - |
- |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.1.2 | 4°C, in presence of sodium borate, stable for 5 days | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.2 | Gln + H2O | - |
Escherichia coli | Glu + NH3 | - |
? |  |
| 3.5.1.2 | L-Gln + hydroxylamine | - |
Escherichia coli | L-gamma-glutamyl hydroxamate + NH3 | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.2 | 4 | - |
low level of activity after partial purification at 4°C in absence of stabilizing ligands, warming at 24°C results in 2fold to 5fold increase in activity | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.2 | 7.1 | 9 | hydrolysis of Gln, glutaminase B | Escherichia coli |
| 3.5.1.2 | 8.3 | 9.2 | formation of gamma-glutamyl hydroxamate from L-Gln and NH2OH | Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.2 | 6 | 11 | pH 6.0: about 55% of maximal activity, pH 11.0: about 55% of maximal activity, hydrolysis of Gln | Escherichia coli |
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