EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.38 | 2-mercaptoethanol | slight activation | Rhodotorula toruloides | |
3.5.1.38 | cysteine | slight activation | Rhodotorula toruloides | |
3.5.1.38 | dithiothreitol | slight activation | Rhodotorula toruloides |
EC Number | General Stability | Organism |
---|---|---|
3.5.1.38 | stabilizing effect by addition of glycine and maintaining the pH to 9.0 | Rhodotorula toruloides |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.38 | 2-Hydroxy-5-nitrobenzyl bromide | 40% inhibition at 10 mM, concentration-dependent, protection against inhibition in presence of substrate | Rhodotorula toruloides | |
3.5.1.38 | Hg2+ | slight inhibition | Rhodotorula toruloides | |
3.5.1.38 | iodoacetamide | slight inhibition | Rhodotorula toruloides | |
3.5.1.38 | N-bromosuccinimide | concentration-dependent inhibition, 40% at 10 mM, protection against inhibition in presence of substrate | Rhodotorula toruloides | |
3.5.1.38 | p-chloromercuribenzoate | slight inhibition | Rhodotorula toruloides | |
3.5.1.38 | Urea | 95% loss of activity in 2.25 M | Rhodotorula toruloides |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.38 | 1.43 | - |
L-asparagine | - |
Rhodotorula toruloides | |
3.5.1.38 | 2.4 | - |
N-glycyl-L-asparagine | - |
Rhodotorula toruloides | |
3.5.1.38 | 6.45 | - |
L-glutamine | - |
Rhodotorula toruloides | |
3.5.1.38 | 8 | - |
N-carbamoyl-L-asparagine | - |
Rhodotorula toruloides | |
3.5.1.38 | 10 | - |
N-acetyl-L-asparagine | - |
Rhodotorula toruloides |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.1.38 | periplasmic space | - |
Rhodotorula toruloides | 42597 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.38 | Ni2+ | slightly enhanced activity | Rhodotorula toruloides |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.38 | 87000 | - |
2 * 87000, SDS-PAGE | Rhodotorula toruloides |
3.5.1.38 | 180000 | - |
gel filtration | Rhodotorula toruloides |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.38 | L-asparagine + H2O | Rhodotorula toruloides | - |
L-aspartate + NH3 | - |
? | |
3.5.1.38 | L-glutamine + H2O | Rhodotorula toruloides | one fifth of the activity on L-asparagine | L-glutamate + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.38 | Rhodotorula toruloides | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.38 | to homogeneity, chromatography, preparative gel electrophoresis | Rhodotorula toruloides |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.1.38 | cell culture | - |
Rhodotorula toruloides | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.38 | 2156 | - |
glutaminase activity | Rhodotorula toruloides |
3.5.1.38 | 10510 | - |
asparaginase activity | Rhodotorula toruloides |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.1.38 | -10°C, lyophilized to dryness in the presence of 50 mM Tris, 250 mM glycine, pH 9.0, 90% activity in 3 months | Rhodotorula toruloides |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.38 | beta-L-aspartyl hydroxylammonium sulfate + H2O | - |
Rhodotorula toruloides | L-Asp + hydroxylammonium sulfate | - |
? | |
3.5.1.38 | gamma-L-glutamyl hydroxylammonium sulfate + H2O | - |
Rhodotorula toruloides | L-glutamate + hydroxylammonium sulfate | - |
? | |
3.5.1.38 | L-asparagine + H2O | - |
Rhodotorula toruloides | L-aspartate + NH3 | - |
? | |
3.5.1.38 | L-asparagine + H2O | not active on D-asparagine | Rhodotorula toruloides | L-aspartate + NH3 | - |
? | |
3.5.1.38 | L-glutamine + H2O | one fifth of the activity on L-asparagine | Rhodotorula toruloides | L-glutamate + NH3 | - |
? | |
3.5.1.38 | N-acetyl-L-asparagine + H2O | - |
Rhodotorula toruloides | N-acetyl-L-aspartate + NH3 | - |
? | |
3.5.1.38 | N-carbamoyl-L-asparagine + H2O | - |
Rhodotorula toruloides | N-carbamoyl-L-aspartate + NH3 | - |
? | |
3.5.1.38 | N-glycyl-L-asparagine + H2O | 60% higher activity than on L-asparagine | Rhodotorula toruloides | N-glycyl-L-aspartate + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.38 | dimer | 2 * 87000, SDS-PAGE | Rhodotorula toruloides |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.38 | 37 | - |
- |
Rhodotorula toruloides |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.38 | 5 | 7 | optimal range for asparaginase activity | Rhodotorula toruloides |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.38 | 7 | - |
complete inactivation | Rhodotorula toruloides |