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Literature summary extracted from

  • Woods, M.J.; Findlater, J.D.; Orsi, B.A.
    Kinetic mechanismen of the aliphatic amidase from Pseudomonas aeruginosa (1979), Biochim. Biophys. Acta, 567, 225-237.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 acetaldehyde
-
 Pseudomonas aeruginosa
3.5.1.4 acetamide non-competitive inhibitor Pseudomonas aeruginosa
3.5.1.4 acetic acid competitive product inhibitor at pH 5.7 Pseudomonas aeruginosa
3.5.1.4 ammonia non-competitive product inhibitor at pH 9.0 Pseudomonas aeruginosa
3.5.1.4 hydroxylamine competitive inhibitor at pH 7.2 Pseudomonas aeruginosa
3.5.1.4 iodoacetamide
-
 Pseudomonas aeruginosa
3.5.1.4 Urea competitive inhibitor at pH 7.2, 90 mM acetamide protectes AI3 amidase against 2.9 mM urea over 2 h, Ki of strain AI3 is 6.6 mM, Ki of strain AIU1N is 41.3 mM and Ki of strain OUCH4 is not known, regaines full activity within 24 h at 37°C in 0.05 M Tris buffer at pH 7.2 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.4 acetohydrazide + H2O Pseudomonas aeruginosa
-
 hydrazine + acetate
-
 ?
3.5.1.4 acetohydroxamate + H2O Pseudomonas aeruginosa
-
 hydroxylamine + acetate
-
 ?
3.5.1.4 hydroxylamine + acylamide Pseudomonas aeruginosa acyltransferase activity acylhydroxamate + NH3
-
 ?
3.5.1.4 N-methylacetamide + H2O Pseudomonas aeruginosa
-
 methylamine + acetate
-
 ?
3.5.1.4 N-methylpropionamide + H2O Pseudomonas aeruginosa
-
 methylamine + propionate
-
 ?
3.5.1.4 propionohydrazide + H2O Pseudomonas aeruginosa
-
 hydrazine + propionate
-
 ?
3.5.1.4 propionohydroxamate + H2O Pseudomonas aeruginosa
-
 hydroxylamine + propionate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Pseudomonas aeruginosa
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.4 a monocarboxylic acid amide + H2O = a monocarboxylate + NH3 hydrolysis of aliphatic amide Pseudomonas aeruginosa
a
3.5.1.4 a monocarboxylic acid amide + H2O = a monocarboxylate + NH3 ping-pong mechanism, substitution mechanism Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 acetohydrazide + H2O
-
 Pseudomonas aeruginosa hydrazine + acetate
-
 ?
3.5.1.4 acetohydroxamate + H2O
-
 Pseudomonas aeruginosa hydroxylamine + acetate
-
 ?
3.5.1.4 hydroxylamine + acylamide acyltransferase activity Pseudomonas aeruginosa acylhydroxamate + NH3
-
 ?
3.5.1.4 N-methylacetamide + H2O
-
 Pseudomonas aeruginosa methylamine + acetate
-
 ?
3.5.1.4 N-methylpropionamide + H2O
-
 Pseudomonas aeruginosa methylamine + propionate
-
 ?
3.5.1.4 propionamide + H2O
-
 Pseudomonas aeruginosa propionate + NH3
-
 ?
3.5.1.4 propionohydrazide + H2O
-
 Pseudomonas aeruginosa hydrazine + propionate
-
 ?
3.5.1.4 propionohydroxamate + H2O
-
 Pseudomonas aeruginosa hydroxylamine + propionate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.4 0.3
-
 N-methylpropionamide hydrolysis Pseudomonas aeruginosa
3.5.1.4 1.08
-
 N-Methylacetamide hydrolysis Pseudomonas aeruginosa
3.5.1.4 9.5
-
 propionohydrazide transferase reaction Pseudomonas aeruginosa
3.5.1.4 10
-
 acetohydrazide hydrolysis Pseudomonas aeruginosa
3.5.1.4 14
-
 Acetohydroxamate hydrolysis Pseudomonas aeruginosa
3.5.1.4 20
-
 acetohydrazide transferase reaction Pseudomonas aeruginosa
3.5.1.4 22
-
 propionohydroxamate hydrolysis Pseudomonas aeruginosa
3.5.1.4 162
-
 acetamide hydrolysis Pseudomonas aeruginosa
3.5.1.4 374
-
 Propionamide hydrolysis Pseudomonas aeruginosa
3.5.1.4 465
-
 Propionamide transferase reaction Pseudomonas aeruginosa
3.5.1.4 790
-
 acetamide transferase reaction Pseudomonas aeruginosa
3.5.1.4 890
-
 N-Methylacetamide transferase reaction Pseudomonas aeruginosa