Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, J.M.; Shimizu, S.; Yamada, H.
    Purification and characterization of a novel enzyme, N-carbamoylsarcosine amidohydrolase, from Pseudomonas putida 77 (1986), J. Biol. Chem., 261, 11832-11839.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.59
-
 Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.59 Ag2+
-
 Pseudomonas putida
3.5.1.59 Cu2+
-
 Pseudomonas putida
3.5.1.59 Hg2+
-
 Pseudomonas putida
3.5.1.59 additional information no inhibition by 2 mM MgCl2, FeSO4, CdCl2, MnCl2, BaCl2, FeCl3, CaCl2, PbCl2, DTT or 2-mercaptoethanol, 1 mM 1,10-phenanthroline, 2,2'-dipyridyl, hydrazine, phenylhydrazine, 8-hydroxyquinoline, 4 mM sarcosine, glycine, N-methyl-DL-valine, N-methyl-DL-alanine, N,N-dimethylglycine, N-methylleucine, methylurea, dimethylamine, trimethylamine or diethylamine Pseudomonas putida
3.5.1.59 NaN3 weak Pseudomonas putida
3.5.1.59 PCMB
-
 Pseudomonas putida
3.5.1.59 Zn2+ weak Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.59 3.2
-
 N-carbamoylsarcosine
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.59 27000
-
 x * 27000, SDS-PAGE Pseudomonas putida
3.5.1.59 75000
-
 HPLC gel filtration Pseudomonas putida
3.5.1.59 88000
-
 gel filtration Pseudomonas putida
3.5.1.59 102000
-
 sedimentation equilibrium method Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.59 N-carbamoylsarcosine + H2O Pseudomonas putida creatine pathway sarcosine + CO2 + NH3
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.59 Pseudomonas putida
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.59 no glycoprotein
-
 Pseudomonas putida

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.59
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.59 0.958
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.59 additional information poor substrates are N-carbamoyl derivatives of DL-tryptophan, DL-phenylalanine, 4-hydroxy-DL-phenylglycine, D-valine, 4-methyl-DL-phenylglycine, DL-2-amino-n-butyric acid, DL-threonine or D-leucine. No substrates are N-carbamoyl derivatives of L-glutamic acid, L-valine, L-tyrosine, L-isoleucine, L-histidine, L-asparagine, DL-aspartic acid, L-serine, DL-methionine or 2-aminoisobutyric acid Pseudomonas putida ?
-
 ?
3.5.1.59 N-carbamoyl-D-alanine + H2O hydrolysis at 7% the rate of N-carbamoylsarcosine Pseudomonas putida ?
-
 ?
3.5.1.59 N-carbamoylglycine + H2O hydrolysis at 10% the rate of N-carbamoylsarcosine Pseudomonas putida ?
-
 ?
3.5.1.59 N-carbamoylsarcosine + H2O best substrate Pseudomonas putida sarcosine + CO2 + NH3
-
 ir
3.5.1.59 N-carbamoylsarcosine + H2O creatine pathway Pseudomonas putida sarcosine + CO2 + NH3
-
 ir
3.5.1.59 N-methyl-N-carbamoyl-D-alanine + H2O hydrolysis at 13% the rate of N-carbamoylsarcosine Pseudomonas putida ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.59 ? x * 27000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.59 37
-
-
 Pseudomonas putida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.59 30 50 about half-maximal activity at 30°C and 50°C Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.59 40
-
 10 min, stable at pH 6-7 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.59 7 8
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.59 5 8.7 about half-maximal activity at pH 5 and 8.7 Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.59 6 7 10 min, stable at 40°C Pseudomonas putida