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Literature summary extracted from

  • Soda, K.; Uchiyama, K.; Ogata, K.
    Metabolism of L-theanine, D-theanine and the related compounds in bacteria. Part II. Purification and properties of the enzyme hydrolyzing L-theanine, D-theanine and the related compounds (1966), Agric. Biol. Chem., 30, 547-557.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.65 CuSO4 10 mM: strong inhibition Pseudomonas aeruginosa
3.5.1.65 EDTA 10 mM: 30% inhibition Pseudomonas aeruginosa
3.5.1.65 HgCl2 1 mM: strong inhibition Pseudomonas aeruginosa
3.5.1.65 iodoacetate 2 mM: strong inhibition, protected partially, about 30%, by preincubation, 15 min, with 20 mM 2-mercaptoethanol Pseudomonas aeruginosa
3.5.1.65 additional information no inhibition by urea, p-chloromercuribenzoate, NaF or KF Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.65 5.8
-
 L-Theanine partial purified enzyme Pseudomonas aeruginosa
3.5.1.65 9.2
-
 D-theanine partial purified enzyme Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.65 additional information no activation by phosphate ions Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.65 N5-ethyl-L-glutamine + H2O Pseudomonas aeruginosa
-
 L-glutamate + ethylamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.65 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.65
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.65 36.83
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.65 D-glutamine + H2O
-
 Pseudomonas aeruginosa D-glutamate + NH3
-
 ?
3.5.1.65 L-glutamine + H2O
-
 Pseudomonas aeruginosa L-glutamate + NH3
-
 ?
3.5.1.65 additional information substrate specificity Pseudomonas aeruginosa ?
-
 ?
3.5.1.65 additional information no activity with L-asparagine, D-asparagine or acetamide Pseudomonas aeruginosa ?
-
 ?
3.5.1.65 N5-ethyl-D-glutamine + H2O D-theanine, probably by the same enzyme like L-theanine Pseudomonas aeruginosa D-glutamate + ethylamine
-
 ?
3.5.1.65 N5-ethyl-L-glutamine + H2O
-
 Pseudomonas aeruginosa L-glutamate + ethylamine
-
 ?
3.5.1.65 theanine + hydroxylamine transferase reaction Pseudomonas aeruginosa gamma-glutamylhydroxamic acid + ethylamine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.65 37
-
 assay at Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.65 55
-
 60 min, stable at over a wide range of pH Pseudomonas aeruginosa
3.5.1.65 55
-
 above 55°C: rapid inactivation of enzyme Pseudomonas aeruginosa
3.5.1.65 60
-
 15 min, rapid inactivation of enzyme Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.65 8
-
 assay at Pseudomonas aeruginosa
3.5.1.65 8.5 9
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.65 5.5 10.5 about half of maximum activity at pH 5.5 and 10.5 Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.65 5 9.5 quite stable between Pseudomonas aeruginosa