Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Martens, M.; Cottenie-Ruysschaert, M.; Hanselaer, R.; De Cooman, L.; Vande Casteele, K.; Van Sumere, C.F.
    Characteristics and specificity of purified N-feruloylglycine amidohydrolase from isolated barley embryos (1988), Phytochemistry, 27, 2465-2475.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.5.1.71 bivalent cations important for stability Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.71 1,10-phenanthroline o-phenanthroline, 10 mM: 97% inhibition, partially reversed by CuCl2, CoCl2 and to a lesser extent by ZnCl2 and MnCl2, 1 mM: 29% inhibition, 0.1 mM: no inhibition. 2.5 mM: 73% inhibition, totally reversed by 0.66 mM ZnCl2, CuCl2 or CoCl2 and to a lesser extent also by nickel and manganous ions Hordeum vulgare
3.5.1.71 1,4-dithiothreitol 2 mM: 100% inhibition Hordeum vulgare
3.5.1.71 2-Iodosobenzoate 5 mM: 100% inhibition Hordeum vulgare
3.5.1.71 2-mercaptoethanol 20 mM: 100% inhibition Hordeum vulgare
3.5.1.71 Co2+ 1 mM: about 37% inhibition Hordeum vulgare
3.5.1.71 CoSO4 1 mM: about 37% inhibition Hordeum vulgare
3.5.1.71 diphenyl-1,5-thiocarbazone dithizone, 10 mM: 79% inhibition Hordeum vulgare
3.5.1.71 EDTA 10 mM: 61% inhibition Hordeum vulgare
3.5.1.71 glutathione reduced glutathione, 50 mM: 100% inhibition Hordeum vulgare
3.5.1.71 HgCl2 0.005 mM: 100% inhibition Hordeum vulgare
3.5.1.71 iodoacetamide 7.5 mM: 100% inhibition Hordeum vulgare
3.5.1.71 iodoacetate 4 mM: 100% inhibition Hordeum vulgare
3.5.1.71 L-cysteine 50 mM: 100% inhibition Hordeum vulgare
3.5.1.71 Metal chelating reagents high concentration, 10 mM Hordeum vulgare
3.5.1.71 additional information kinetics of inhibition; no inhibition by cystine and disodium maleate Hordeum vulgare
3.5.1.71 N-ethylmaleimide 1 mM: 100% inhibition Hordeum vulgare
3.5.1.71 N-feruloyl-Gly-L-LeuOH 5 mM: potent reversible inhibitor, kinetics of inhibition. Ki: 0.3 mM Hordeum vulgare
3.5.1.71 N-feruloyl-Gly-L-PheOH 5 mM: potent reversible inhibitor, kinetics of inhibition. Ki: 0.042 mM Hordeum vulgare
3.5.1.71 N-feruloyldipeptides potent reversible inhibitor Hordeum vulgare
3.5.1.71 p-chloromercuribenzoate 0.05 mM: 100% inhibition Hordeum vulgare
3.5.1.71 sulfhydryl reagents
-
 Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.71 additional information
-
 additional information kinetic data, kinetic studies Hordeum vulgare
3.5.1.71 0.084
-
 N-feruloylglycine Eadie-Hofstee plot Hordeum vulgare
3.5.1.71 0.085
-
 N-feruloylglycine
-
 Hordeum vulgare
3.5.1.71 0.086
-
 N-feruloylglycine Lineweaver-Burk plot Hordeum vulgare
3.5.1.71 0.133
-
 N-Sinapoylglycine
-
 Hordeum vulgare
3.5.1.71 0.217
-
 N-Feruloyl-L-alanine
-
 Hordeum vulgare
3.5.1.71 0.385
-
 N-p-Coumaroylglycine
-
 Hordeum vulgare
3.5.1.71 0.426
-
 N-cinnamoylglycine
-
 Hordeum vulgare
3.5.1.71 0.833
-
 N-Dihydroferuloylglycine
-
 Hordeum vulgare
3.5.1.71 0.909
-
 N-Feruloyl-D,L-aspartic acid
-
 Hordeum vulgare
3.5.1.71 1.11
-
 N-Vanilloyl-1-glycine
-
 Hordeum vulgare
3.5.1.71 1.18
-
 N-4-Hydroxybenzoylglycine
-
 Hordeum vulgare
3.5.1.71 2
-
 N-benzoylglycine
-
 Hordeum vulgare

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.71 Bivalent cations important for proper function Hordeum vulgare
3.5.1.71 additional information no activation by Co2+, 1 mM CoSO4 even inhibitory Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.71 155000
-
 gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.71 N-feruloylglycine + H2O Hordeum vulgare best substrate ferulate + glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.71 Hordeum vulgare
-
 barley
-
3.5.1.71 Hordeum vulgare
-
 cv Zephyr
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.71
-
 Hordeum vulgare

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.71 N-feruloylglycine + H2O = ferulate + glycine thermodynamic data Hordeum vulgare
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.71 embryo
-
 Hordeum vulgare
-
3.5.1.71 seed
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.71 additional information
-
-
 Hordeum vulgare
3.5.1.71 0.022
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.71 additional information substrate specificity Hordeum vulgare ?
-
 ?
3.5.1.71 additional information N-syringoylglycine is only poorly hydrolyzed, N-galloylglycine, N-protocatechuoylglycine and N-caffeoylglycine are hydrolyzed, but due to oxidation and other reactions the activity of the enzyme with the corresponding substrate cannot be accurately measured Hordeum vulgare ?
-
 ?
3.5.1.71 additional information no activity with N-acetylamino acids, Azocoll, N-carbobenzoxy-L-Phe-L-AlaOH, Nalpha-benzoyl-L-Arg-ethyl ester, N-feruloyl-Gly-L-PheOH, N-feruloyl-Gly-L-LeuOH, L-Leu-p-nitroanilide, Nalpha-benzoyl-D,L-Arg-p-nitroanilide, N-L-leucyl-L-tyrosine, N-L-alanyl-L-proline, N-L-phenylalanylglycine, N-L-phenylalanyl-L-alanine, N-formyl-amino acids, N-formyl-L-aspartic acid, N-formyl-L-methionine and N-feruloylamide Hordeum vulgare ?
-
 ?
3.5.1.71 additional information no deformylase or amidase activity Hordeum vulgare ?
-
 ?
3.5.1.71 additional information hydrolyses range of L-amino acids from the cinnamoyl, substituted cinnamoyl and benzoyl series Hordeum vulgare ?
-
 ?
3.5.1.71 N-4-hydroxybenzoylglycine + H2O
-
 Hordeum vulgare 4-hydroxybenzoate + glycine
-
 ?
3.5.1.71 N-benzoylglycine + H2O
-
 Hordeum vulgare benzoate + glycine
-
 ?
3.5.1.71 N-cinnamoylglycine + H2O
-
 Hordeum vulgare cinnamate + glycine
-
 ?
3.5.1.71 N-dihydroferuloylglycine + H2O
-
 Hordeum vulgare dihydroferulate + glycine
-
 ?
3.5.1.71 N-feruloyl-D,L-aspartic acid + H2O
-
 Hordeum vulgare ferulate + D,L-aspartate
-
 ?
3.5.1.71 N-feruloyl-L-alanine + H2O
-
 Hordeum vulgare ferulate + L-alanine
-
 ?
3.5.1.71 N-feruloylglycine + H2O best substrate Hordeum vulgare ferulate + glycine
-
 ?
3.5.1.71 N-p-coumaroylglycine + H2O
-
 Hordeum vulgare p-coumarate + glycine
-
 ?
3.5.1.71 N-sinapoylglycine + H2O
-
 Hordeum vulgare sinapate + glycine
-
 ?
3.5.1.71 N-syringoylglycine + H2O only poorly hydrolyzed Hordeum vulgare syringate + glycine
-
 ?
3.5.1.71 N-vanilloyl-1-glycine + H2O
-
 Hordeum vulgare 4-hydroxy-3-methoxybenzoate + glycine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.71 N-FGAH
-
 Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.71 30
-
 assay at Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.71 8
-
-
 Hordeum vulgare

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.71 0.042
-
 N-feruloyl-Gly-L-PheOH 5 mM: potent reversible inhibitor, kinetics of inhibition. Hordeum vulgare
3.5.1.71 0.3
-
 N-feruloyl-Gly-L-LeuOH 5 mM: potent reversible inhibitor, kinetics of inhibition Hordeum vulgare