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Literature summary extracted from

  • Brooks, K.P.; Jones, E.A.; Kim, B.D.; Sander, E.G.
    Bovine liver dihydropyrimidine amidohydrolase: Purification, properties, and characterization as a zinc metalloenzyme (1983), Arch. Biochem. Biophys., 226, 469-483.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.2 2,6-dipicolinic acid
-
 Bos taurus
3.5.2.2 4-nitrobenzene sulfonamide
-
 Bos taurus
3.5.2.2 8-Hydroxyquinoline-5-sulfonic acid
-
 Bos taurus
3.5.2.2 o-phenanthroline
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.2 0.008
-
 5,6-dihydrouracil
-
 Bos taurus
3.5.2.2 0.015
-
 Dihydrothymine
-
 Bos taurus
3.5.2.2 0.015
-
 beta-ureidopropionate 0.1 mM Bos taurus
3.5.2.2 0.018
-
 5-iododihydrouracil
-
 Bos taurus
3.5.2.2 0.02
-
 5-bromodihydrouracil
-
 Bos taurus
3.5.2.2 0.028
-
 beta-Ureidoisobutyrate 0.1 mM Bos taurus
3.5.2.2 0.069
-
 beta-Ureidoisobutyrate 0.1-3.25 mM Bos taurus
3.5.2.2 0.079
-
 beta-ureidopropionate 0.1-5 mM Bos taurus
3.5.2.2 50
-
 hydantoin
-
 Bos taurus
3.5.2.2 62.5
-
 5-methylhydantoin
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.2 Zn2+ essential, metalloenzyme Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 56500
-
 4 * 56500, SDS-PAGE Bos taurus
3.5.2.2 226000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.2 5,6-dihydropyrimidine + H2O Bos taurus
-
 beta-ureido acid
-
 r
3.5.2.2 5,6-dihydrothymine + H2O Bos taurus
-
 ?
-
 r
3.5.2.2 5,6-dihydrouracil + H2O Bos taurus
-
 3-ureidopropionate
-
 ?
3.5.2.2 hydantoin + H2O Bos taurus
-
 N-carbamoyl glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Bos taurus
-
 beef
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.2 kidney
-
 Bos taurus
-
3.5.2.2 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.2 10.1
-
-
 Bos taurus
3.5.2.2 10.4
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.5.2.2 4°C, stable in dilute Tris buffer pH 7.5 for several days with no loss of activity, purified enzyme loses up to 50% activity after 6 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydropyrimidine + H2O
-
 Bos taurus beta-ureido acid
-
 r
3.5.2.2 5,6-dihydrothymine + H2O
-
 Bos taurus ?
-
 r
3.5.2.2 5,6-dihydrouracil + H2O
-
 Bos taurus 3-ureidopropionate
-
 ?
3.5.2.2 5-bromo-5,6-dihydrouracil + H2O
-
 Bos taurus ?
-
 r
3.5.2.2 5-iodo-5,6-dihydrouracil + H2O
-
 Bos taurus ?
-
 ?
3.5.2.2 beta-ureidoisobutyrate
-
 Bos taurus dihydrothymine + H2O
-
 r
3.5.2.2 D-5-methylhydantoin + H2O
-
 Bos taurus N-carbamoyl-D-alanine
-
 ?
3.5.2.2 DL-5-methylhydantoin
-
 Bos taurus ?
-
 ?
3.5.2.2 hydantoin + H2O
-
 Bos taurus N-carbamoyl glycine
-
 ?
3.5.2.2 additional information L-dihydroorotic acid is no substrate Bos taurus ?
-
 ?
3.5.2.2 N-carbamoyl beta-alanine + H2O
-
 Bos taurus 5,6-dihydrouracil
-
 r
3.5.2.2 succinimide + H2O
-
 Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.2 tetramer 4 * 56500, SDS-PAGE Bos taurus