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Literature summary extracted from

  • Luksa, V.; Starkuviene, V.; Starkuviene, B.; Dagys, R.
    Purification and characterization of the D-hydantoinase from Bacillus circulans (1997), Appl. Biochem. Biotechnol., 62, 219-230.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.2.2 synthesis commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals Geobacillus stearothermophilus
3.5.2.2 synthesis commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals Niallia circulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.2 p-hydroxymercuribenzoate
-
 Geobacillus stearothermophilus
3.5.2.2 p-hydroxymercuribenzoate
-
 Niallia circulans
3.5.2.2 p-hydroxymercuribenzoate
-
 Pseudomonas fluorescens
3.5.2.2 p-hydroxymercuribenzoate
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.2 4.9
-
 DL-5-isobutylhydantoin
-
 Niallia circulans
3.5.2.2 5.8
-
 allantoin
-
 Niallia circulans
3.5.2.2 6.8
-
 DL-5-benzylhydantoin
-
 Niallia circulans
3.5.2.2 12.8
-
 DL-5-butylhydantoin
-
 Niallia circulans
3.5.2.2 17.2
-
 DL-5-isopropylhydantoin
-
 Niallia circulans
3.5.2.2 25.6
-
 DL-5-propylhydantoin
-
 Niallia circulans
3.5.2.2 27.8
-
 DL-5-methylhydantoin
-
 Niallia circulans
3.5.2.2 40.7
-
 DL-5-(2-methylthioethyl)hydantoin
-
 Niallia circulans
3.5.2.2 58.8
-
 DL-5-phenyllhydantoin
-
 Niallia circulans
3.5.2.2 105.4
-
 hydantoin
-
 Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 53000
-
 4 * 53000, SDS-PAGE Niallia circulans
3.5.2.2 54000
-
 4 * 54000, gel filtration, denaturing conditions Niallia circulans
3.5.2.2 212000
-
 gel filtration Niallia circulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.2 hydantoin + H2O Brevibacillus brevis
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Geobacillus stearothermophilus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas fluorescens
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas putida
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Niallia circulans
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Arthrobacter crystallopoietes
-
 N-carbamoyl glycine
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.5.2.2 SDS
-
 Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Agrobacterium sp.
-
-
-
3.5.2.2 Agrobacterium sp. IP-I 671
-
-
-
3.5.2.2 Arthrobacter crystallopoietes
-
-
-
3.5.2.2 Brevibacillus brevis
-
 L-specific hydantoinase
-
3.5.2.2 Geobacillus stearothermophilus
-
-
-
3.5.2.2 Niallia circulans
-
-
-
3.5.2.2 Pseudomonas fluorescens
-
-
-
3.5.2.2 Pseudomonas putida
-
-
-
3.5.2.2 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2
-
 Niallia circulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.2 43.8
-
-
 Niallia circulans

Storage Stability

EC Number Storage Stability Organism
3.5.2.2 -20°C, 5 mM Tris buffer pH 8.5, 0.05 mM MnCl2, 50% initial activity left after 6 months Niallia circulans
3.5.2.2 4°C, 5 mM Tris buffer pH 8.5, 0.05 mM MnCl2, retains full activity 1 month Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 allantoin + H2O
-
 Niallia circulans ?
-
 ?
3.5.2.2 allantoin + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 allantoin + H2O
-
 Agrobacterium sp. IP-I 671 ?
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Niallia circulans N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Arthrobacter crystallopoietes N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium sp. IP-I 671 N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Niallia circulans ?
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Arthrobacter crystallopoietes ?
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Agrobacterium sp. IP-I 671 ?
-
 ?
3.5.2.2 DL-5-butylhydantoin
-
 Niallia circulans ?
-
 ?
3.5.2.2 DL-5-isobutyl-hydantoin + H2O
-
 Niallia circulans ?
-
 ?
3.5.2.2 DL-5-isobutyl-hydantoin + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 DL-5-isobutyl-hydantoin + H2O
-
 Agrobacterium sp. IP-I 671 ?
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Niallia circulans N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Agrobacterium sp. IP-I 671 N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-methylhydantoin
-
 Niallia circulans ?
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Niallia circulans N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Arthrobacter crystallopoietes N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-propylhydantoin
-
 Niallia circulans ?
-
 ?
3.5.2.2 hydantoin + H2O
-
 Brevibacillus brevis N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Niallia circulans N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Arthrobacter crystallopoietes N-carbamoyl glycine
-
 ?
3.5.2.2 additional information
-
 Pseudomonas putida ?
-
 ?
3.5.2.2 additional information 5,5-dimethylhydantoin is no substrate Niallia circulans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.2 tetramer
-
 Geobacillus stearothermophilus
3.5.2.2 tetramer
-
 Pseudomonas fluorescens
3.5.2.2 tetramer
-
 Arthrobacter crystallopoietes
3.5.2.2 tetramer 4 * 53000, SDS-PAGE Niallia circulans
3.5.2.2 tetramer 4 * 54000, gel filtration, denaturing conditions Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.2 75
-
 DL-5-isopropylhydantoin as substrate Niallia circulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.2 60
-
-
 Geobacillus stearothermophilus
3.5.2.2 60
-
-
 Pseudomonas putida
3.5.2.2 60
-
 stable up to, retains full activity on heating at 40°C for 8 h, retains 75% original activity at 62.5°C for 8 h Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.2 8 10
-
 Niallia circulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.2 8.5 9.5 most stable in this range Niallia circulans